Detailed information of TA system TA221834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1118853..1119403
• Replicon: chromosome
• Accession: NZ_CP085814
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Plusle

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: M7RJ32
• Locus tag: LJF38_RS05300
• Protein ID: WP_001199743.1
• Coordinates: 1119095..1119403 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7RP97
• Locus tag: LJF38_RS05295
• Protein ID: WP_001118105.1
• Coordinates: 1118853..1119092 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF38_RS05260 (1114292)	1114292..1115323	-	1032	WP_000453347.1	L-idonate 5-dehydrogenase	-
LJF38_RS05265 (1115540)	1115540..1116070	+	531	WP_000896758.1	gluconokinase	-
LJF38_RS05270 (1116098)	1116098..1117117	-	1020	WP_000152558.1	NAD(P)-dependent alcohol dehydrogenase	-
LJF38_RS05285 (1117874)	1117874..1118419	+	546	WP_223151225.1	helix-turn-helix domain-containing protein	-
LJF38_RS05290 (1118496)	1118496..1118744	+	249	WP_000168388.1	ribbon-helix-helix domain-containing protein	-
LJF38_RS05295 (1118853)	1118853..1119092	+	240	WP_001118105.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LJF38_RS05300 (1119095)	1119095..1119403	+	309	WP_001199743.1	CcdB family protein	Toxin
LJF38_RS05305 (1119809)	1119809..1119949	+	141	Protein_1020	Arm DNA-binding domain-containing protein	-
LJF38_RS05310 (1119981)	1119981..1121114	-	1134	Protein_1021	IS3 family transposase	-
LJF38_RS05315 (1121437)	1121437..1121967	+	531	WP_000909461.1	SEF14 fimbria major subunit SefA	-
LJF38_RS05320 (1122221)	1122221..1122832	+	612	WP_001706282.1	SEF14/SEF18 fimbria chaperone SefB	-
LJF38_RS05325 (1122849)	1122849..1123490	+	642	WP_227798720.1	FimD/PapC N-terminal domain-containing protein	-
LJF38_RS23055 (1123427)	1123427..1123849	+	423	WP_265328806.1	fimbria/pilus outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	1117919..1121022	3103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11845.67 Da
• Isoelectric Point: 6.7228

>T221834 WP_001199743.1 NZ_CP085814:1119095-1119403 [Salmonella enterica subsp. enterica serovar Enteritidis]
MQYMVYRNKGNSKAYPYLLDVQSDIIDELHTRMVIPLFPVSRLVNNPVKRLTPTLNVEGNDYLVMTHEMASIRLSQIGDE
VMDVRSHRQTIKNALDFIFDGF

Nucleotide

• Length: 309 bp

>T221834 NZ_CP117603:22806-23111 [Escherichia albertii]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGACAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8719.90 Da
• Isoelectric Point: 6.4633

>AT221834 WP_001118105.1 NZ_CP085814:1118853-1119092 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPGMSVRHKKTVSVTLEPALLQQARDAGINLSAVLTAALKEEISATGVERWKAENRAGLQELNRITDEHGLLSDDYRTF

Nucleotide

• Length: 240 bp

>AT221834 NZ_CP117603:22586-22804 [Escherichia albertii]
ATGAAGCAGCGCATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A6C6Z9V9

Antitoxin

• AlphaFold DB: A0A5H9SZK8