Detailed information of TA system TA221642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 117651..118253
• Replicon: chromosome
• Accession: NZ_CP085805
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Azumarill

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M7S4R6
• Locus tag: LJF48_RS00540
• Protein ID: WP_001159635.1
• Coordinates: 117942..118253 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJF48_RS00535
• Protein ID: WP_000362050.1
• Coordinates: 117651..117941 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF48_RS00520 (115144)	115144..116046	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
LJF48_RS00525 (116043)	116043..116678	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJF48_RS00530 (116675)	116675..117604	+	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
LJF48_RS00535 (117651)	117651..117941	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
LJF48_RS00540 (117942)	117942..118253	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
LJF48_RS00545 (118471)	118471..119400	+	930	WP_001127705.1	alpha/beta hydrolase	-
LJF48_RS00550 (119486)	119486..119797	+	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	-
LJF48_RS00555 (119794)	119794..120240	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	-
LJF48_RS00560 (120255)	120255..121196	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJF48_RS00565 (121241)	121241..121678	-	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
LJF48_RS00570 (121675)	121675..122547	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LJF48_RS00575 (122541)	122541..123140	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12340.30 Da
• Isoelectric Point: 9.4460

>T221642 WP_001159635.1 NZ_CP085805:c118253-117942 [Salmonella enterica subsp. enterica serovar Enteritidis]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T221642 NZ_CP117558:2330260-2330538 [Escherichia albertii]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCATTAAGCGGCGATCGCGATGGCATCTGGGCAATATCTGTATCAGGCAACTGGCGAATCACGTTTGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT221642 WP_000362050.1 NZ_CP085805:c117941-117651 [Salmonella enterica subsp. enterica serovar Enteritidis]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT221642 NZ_CP117558:2330538-2330822 [Escherichia albertii]
ATGAAAATGGTCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGACTGTTGACCGGAAAAGCGGCTTTGACGCCAGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCTGAG
AAAACGGTGGATGTGTCGCGGTTGCGCCGTCTGGTGACGCAATAA

Structures

Toxin

• AlphaFold DB: A0A3V2JK97