Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1948548..1949161 Replicon chromosome
Accession NC_009525
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag MRA_RS09100 Protein ID WP_003408465.1
Coordinates 1948548..1948937 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag MRA_RS09105 Protein ID WP_003408469.1
Coordinates 1948934..1949161 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRA_RS09065 1944177..1945091 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
MRA_RS09070 1945094..1945924 + 831 WP_003916848.1 cyclase family protein -
MRA_RS09075 1945924..1946274 + 351 WP_003898986.1 cupin domain-containing protein -
MRA_RS09080 1946327..1947145 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
MRA_RS09085 1947159..1947938 + 780 WP_003408460.1 IclR family transcriptional regulator -
MRA_RS09100 1948548..1948937 - 390 WP_003408465.1 PIN domain-containing protein Toxin
MRA_RS09105 1948934..1949161 - 228 WP_003408469.1 antitoxin Antitoxin
MRA_RS09110 1949379..1950863 + 1485 WP_003408473.1 carboxylase -
MRA_RS09115 1950860..1952107 + 1248 WP_003408476.1 serine hydrolase -
MRA_RS09120 1952150..1952569 - 420 WP_003408483.1 hypothetical protein -
MRA_RS09125 1952559..1953269 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T22161 WP_003408465.1 NC_009525:c1948937-1948548 [Mycobacterium tuberculosis H37Ra]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T22161 NC_009525:c1948937-1948548 [Mycobacterium tuberculosis H37Ra]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT22161 WP_003408469.1 NC_009525:c1949161-1948934 [Mycobacterium tuberculosis H37Ra]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT22161 NC_009525:c1949161-1948934 [Mycobacterium tuberculosis H37Ra]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References