Detailed information of TA system TA221586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4058275..4059029
• Replicon: chromosome
• Accession: NZ_CP085802
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Wailmer

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5RFE2
• Locus tag: LJF51_RS19855
• Protein ID: WP_000558168.1
• Coordinates: 4058275..4058586 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJF51_RS19860
• Protein ID: WP_001259009.1
• Coordinates: 4058583..4059029 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF51_RS19825 (4053933)	4053933..4054835	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
LJF51_RS19830 (4054832)	4054832..4055467	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJF51_RS19835 (4055464)	4055464..4056393	+	930	WP_227798835.1	formate dehydrogenase accessory protein FdhE	-
LJF51_RS19840 (4056440)	4056440..4056730	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
LJF51_RS19845 (4056731)	4056731..4057042	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
LJF51_RS19850 (4057260)	4057260..4058189	+	930	WP_001127705.1	alpha/beta hydrolase	-
LJF51_RS19855 (4058275)	4058275..4058586	+	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	Toxin
LJF51_RS19860 (4058583)	4058583..4059029	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
LJF51_RS19865 (4059044)	4059044..4059985	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJF51_RS19870 (4060030)	4060030..4060467	-	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
LJF51_RS19875 (4060464)	4060464..4061336	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LJF51_RS19880 (4061330)	4061330..4061929	-	600	WP_000965695.1	glucose-1-phosphatase	-
LJF51_RS19885 (4062120)	4062120..4062923	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
LJF51_RS19890 (4062957)	4062957..4063853	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12324.26 Da
• Isoelectric Point: 9.3143

>T221586 WP_000558168.1 NZ_CP085802:4058275-4058586 [Salmonella enterica subsp. enterica serovar Enteritidis]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTLTQPEEMKRYIPSLDNFKHRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYDRGNKE

Nucleotide

• Length: 312 bp

>T221586 NZ_CP117511:c2545648-2545547 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT221586 WP_001259009.1 NZ_CP085802:4058583-4059029 [Salmonella enterica subsp. enterica serovar Enteritidis]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT221586 NZ_CP117511:2545428-2545602 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTT
TTGAATCTTAACCGTTCGGCCTACGAAGCTTGTGAACGGTTATTTTTTATTGTTTTTCGTTGCTTCAAGGATACCGAAAA
TCAGTAGTGCAACAA

Structures

Toxin

• AlphaFold DB: A0A1R2S541