Detailed information of TA system TA022154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1240726..1241294
• Replicon: chromosome
• Accession: NC_009525
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: MRA_RS05935
• Protein ID: WP_003405865.1
• Coordinates: 1240920..1241294 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: MRA_RS05930
• Protein ID: WP_003405863.1
• Coordinates: 1240726..1240923 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRA_RS05910	1236767..1237405	-	639	WP_003898730.1	lipid droplet-associated protein	-
MRA_RS05915	1237495..1238502	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
MRA_RS05920	1238519..1239502	-	984	WP_003898731.1	hypothetical protein	-
MRA_RS05925	1239565..1240638	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
MRA_RS05930	1240726..1240923	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MRA_RS05935	1240920..1241294	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
MRA_RS05940	1241497..1242195	+	699	WP_003898733.1	hypothetical protein	-
MRA_RS05945	1242313..1242498	+	186	WP_003901093.1	hypothetical protein	-
MRA_RS05950	1242425..1242700	-	276	WP_003405867.1	hypothetical protein	-
MRA_RS05955	1242943..1243266	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
MRA_RS05960	1243281..1243991	-	711	WP_003911399.1	hypothetical protein	-
MRA_RS22605	1244013..1244222	+	210	WP_003911400.1	hypothetical protein	-
MRA_RS05970	1244174..1244881	-	708	Protein_1163	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T22154 WP_003405865.1 NC_009525:1240920-1241294 [Mycobacterium tuberculosis H37Ra]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T22154 NC_009525:1240920-1241294 [Mycobacterium tuberculosis H37Ra]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT22154 WP_003405863.1 NC_009525:1240726-1240923 [Mycobacterium tuberculosis H37Ra]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT22154 NC_009525:1240726-1240923 [Mycobacterium tuberculosis H37Ra]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07