Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 754290..754923 Replicon chromosome
Accession NC_009525
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID G0TQD6
Locus tag MRA_RS03465 Protein ID WP_003403365.1
Coordinates 754290..754673 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ56
Locus tag MRA_RS03470 Protein ID WP_003403368.1
Coordinates 754768..754923 (-) Length 52 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRA_RS03440 749582..750118 + 537 WP_003403341.1 50S ribosomal protein L10 -
MRA_RS03445 750155..750547 + 393 WP_003403353.1 50S ribosomal protein L7/L12 -
MRA_RS03450 750540..751235 - 696 WP_003403355.1 TetR/AcrR family transcriptional regulator -
MRA_RS03455 751306..752811 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
MRA_RS03460 752892..753902 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
MRA_RS03465 754290..754673 - 384 WP_003403365.1 ribonuclease VapC6 Toxin
MRA_RS03470 754768..754923 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MRA_RS03475 754999..755715 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
MRA_RS03480 755991..756299 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
MRA_RS03485 756286..756531 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family -
MRA_RS03490 756641..757078 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
MRA_RS03495 757075..757329 - 255 WP_003911263.1 antitoxin VapB7 -
MRA_RS03500 757443..759806 + 2364 WP_003898547.1 arylsulfatase AtsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-44)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13959.73 Da        Isoelectric Point: 6.0803

>T22145 WP_003403365.1 NC_009525:c754673-754290 [Mycobacterium tuberculosis H37Ra]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Download         Length: 384 bp

>T22145 NC_009525:c754673-754290 [Mycobacterium tuberculosis H37Ra]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 52 a.a.        Molecular weight: 5872.72 Da        Isoelectric Point: 5.7734

>AT22145 WP_003403368.1 NC_009525:c754923-754768 [Mycobacterium tuberculosis H37Ra]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Download         Length: 156 bp

>AT22145 NC_009525:c754923-754768 [Mycobacterium tuberculosis H37Ra]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQD6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSF8

References