Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 163304..164927 Replicon plasmid p49589CZ_VIM
Accession NZ_CP085773
Organism Enterobacter hormaechei strain 49589CZ

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag LJU37_RS24610 Protein ID WP_000088044.1
Coordinates 163602..164927 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag LJU37_RS24605 Protein ID WP_012477377.1
Coordinates 163304..163597 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU37_RS24585 (LJU37_24585) 159103..159495 + 393 WP_000341066.1 hypothetical protein -
LJU37_RS24590 (LJU37_24590) 160154..160810 + 657 WP_000462754.1 trypsin-like serine protease -
LJU37_RS24595 (LJU37_24595) 161013..161510 + 498 WP_000340139.1 membrane protein -
LJU37_RS24600 (LJU37_24600) 161515..162903 + 1389 WP_000083579.1 hypothetical protein -
LJU37_RS24605 (LJU37_24605) 163304..163597 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
LJU37_RS24610 (LJU37_24610) 163602..164927 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
LJU37_RS24615 (LJU37_24615) 164988..165194 + 207 WP_000134171.1 hypothetical protein -
LJU37_RS24620 (LJU37_24620) 165295..165705 + 411 WP_000985909.1 hypothetical protein -
LJU37_RS24625 (LJU37_24625) 165718..166533 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
LJU37_RS24630 (LJU37_24630) 166786..167211 + 426 WP_000278471.1 hypothetical protein -
LJU37_RS24635 (LJU37_24635) 167760..168068 + 309 WP_001224686.1 hypothetical protein -
LJU37_RS24640 (LJU37_24640) 168084..168401 + 318 WP_012477379.1 hypothetical protein -
LJU37_RS24645 (LJU37_24645) 168583..169587 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaVIM-4 / aac(6')-Il / aac(3)-Ib / qacE / sul1 / qnrA1 / tet(B) - 1..294454 294454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T221267 WP_000088044.1 NZ_CP085773:163602-164927 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T221267 NZ_CP117368:c14266-13916 [Salmonella enterica subsp. enterica serovar Dublin]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT221267 WP_012477377.1 NZ_CP085773:163304-163597 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT221267 NZ_CP117368:c13911-13609 [Salmonella enterica subsp. enterica serovar Dublin]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References