Detailed information of TA system TA221250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2079262..2079908
• Replicon: chromosome
• Accession: NZ_CP085767
• Organism: Enterobacter hormaechei strain 49589CZ

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LJU37_RS09910
• Protein ID: WP_047050720.1
• Coordinates: 2079558..2079908 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A7H8UAV4
• Locus tag: LJU37_RS09905
• Protein ID: WP_045335986.1
• Coordinates: 2079262..2079561 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU37_RS09880 (LJU37_09880)	2074730..2075314	-	585	WP_023299002.1	fimbrial protein	-
LJU37_RS09885 (LJU37_09885)	2075367..2075969	-	603	WP_047050717.1	LuxR C-terminal-related transcriptional regulator	-
LJU37_RS09890 (LJU37_09890)	2076804..2077592	+	789	WP_045329485.1	winged helix-turn-helix domain-containing protein	-
LJU37_RS09895 (LJU37_09895)	2077594..2078073	+	480	WP_047050718.1	hypothetical protein	-
LJU37_RS09900 (LJU37_09900)	2078316..2079197	+	882	WP_047050719.1	helix-turn-helix domain-containing protein	-
LJU37_RS09905 (LJU37_09905)	2079262..2079561	-	300	WP_045335986.1	XRE family transcriptional regulator	Antitoxin
LJU37_RS09910 (LJU37_09910)	2079558..2079908	-	351	WP_047050720.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LJU37_RS09915 (LJU37_09915)	2080063..2080433	-	371	Protein_1922	hydroxyacid dehydrogenase	-
LJU37_RS09920 (LJU37_09920)	2080536..2080877	-	342	WP_006812446.1	SymE family type I addiction module toxin	-
LJU37_RS09925 (LJU37_09925)	2081014..2081190	+	177	WP_071962261.1	toxin-antitoxin system HicB family antitoxin	-
LJU37_RS09935 (LJU37_09935)	2083404..2084882	+	1479	WP_032620715.1	SulP family inorganic anion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2076804..2101231	24427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13497.59 Da
• Isoelectric Point: 8.8416

>T221250 WP_047050720.1 NZ_CP085767:c2079908-2079558 [Enterobacter hormaechei]
MWTVLFSKVFEQWLLEQEDGLQEKVLADLLNLQNYGPRLPRPYADTVKGSQYKNMKELRIQYAGRPVRAFFAFDPVRQAI
VLCAGDKRNDKTFYEKLIRIADAEFTLHLTSLEAAK

Nucleotide

• Length: 351 bp

>T221250 NZ_CP117366:c14266-13916 [Salmonella enterica subsp. enterica serovar Newport]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11129.87 Da
• Isoelectric Point: 6.4714

>AT221250 WP_045335986.1 NZ_CP085767:c2079561-2079262 [Enterobacter hormaechei]
VKTLNEVIASRSPESQQRIQEMAEEMFLETGLQLLREELQLSQKHVADLMGVSQPAVTQLEQRGNDLKIATLKRYIEAMG
GKLSINVELPTGKRVAFHL

Nucleotide

• Length: 300 bp

>AT221250 NZ_CP117366:c13911-13609 [Salmonella enterica subsp. enterica serovar Newport]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7H8UAV4