Detailed information of TA system TA221247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1891709..1892325
• Replicon: chromosome
• Accession: NZ_CP085767
• Organism: Enterobacter hormaechei strain 49589CZ

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LJU37_RS08995
• Protein ID: WP_077266491.1
• Coordinates: 1891709..1892080 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: LJU37_RS09000
• Protein ID: WP_015569912.1
• Coordinates: 1892083..1892325 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU37_RS08980 (LJU37_08980)	1889209..1890111	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
LJU37_RS08985 (LJU37_08985)	1890108..1890743	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
LJU37_RS08990 (LJU37_08990)	1890740..1891669	+	930	WP_022652077.1	formate dehydrogenase accessory protein FdhE	-
LJU37_RS08995 (LJU37_08995)	1891709..1892080	-	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJU37_RS09000 (LJU37_09000)	1892083..1892325	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
LJU37_RS09005 (LJU37_09005)	1892524..1893444	+	921	WP_047051644.1	alpha/beta hydrolase	-
LJU37_RS09010 (LJU37_09010)	1893453..1894394	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
LJU37_RS09015 (LJU37_09015)	1894439..1894876	-	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
LJU37_RS09020 (LJU37_09020)	1894873..1895754	-	882	WP_003861949.1	virulence factor BrkB family protein	-
LJU37_RS09025 (LJU37_09025)	1895748..1896347	-	600	WP_032649898.1	glucose-1-phosphatase	-
LJU37_RS09030 (LJU37_09030)	1896466..1897266	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T221247 WP_077266491.1 NZ_CP085767:c1892080-1891709 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T221247 NZ_CP117365:c4140629-4140345 [Salmonella enterica subsp. enterica serovar Newport]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATACAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT221247 WP_015569912.1 NZ_CP085767:c1892325-1892083 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT221247 NZ_CP117365:c4140861-4140619 [Salmonella enterica subsp. enterica serovar Newport]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3