Detailed information of TA system TA221183

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 275785..276464
• Replicon: plasmid p60214CZ_VIM
• Accession: NZ_CP085747
• Organism: Enterobacter hormaechei strain 60214CZ

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: J7FU23
• Locus tag: LJU47_RS24925
• Protein ID: WP_015063033.1
• Coordinates: 275785..276126 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: J7FPK5
• Locus tag: LJU47_RS24930
• Protein ID: WP_015063034.1
• Coordinates: 276147..276464 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU47_RS24885 (LJU47_24885)	271003..271233	-	231	WP_022652119.1	hypothetical protein	-
LJU47_RS24890 (LJU47_24890)	271271..271729	-	459	WP_058658228.1	RES family NAD+ phosphorylase	-
LJU47_RS24895 (LJU47_24895)	271726..272172	-	447	WP_022652121.1	DUF2384 domain-containing protein	-
LJU47_RS24900 (LJU47_24900)	272249..272557	-	309	WP_022652122.1	hypothetical protein	-
LJU47_RS24905 (LJU47_24905)	272733..273473	-	741	WP_227793215.1	DNA methyltransferase	-
LJU47_RS24910 (LJU47_24910)	273458..274000	-	543	WP_227793216.1	winged helix-turn-helix domain-containing protein	-
LJU47_RS24915 (LJU47_24915)	273987..274598	-	612	WP_022652124.1	hypothetical protein	-
LJU47_RS24920 (LJU47_24920)	274660..275295	-	636	WP_015063032.1	hypothetical protein	-
LJU47_RS24925 (LJU47_24925)	275785..276126	-	342	WP_015063033.1	TA system toxin CbtA family protein	Toxin
LJU47_RS24930 (LJU47_24930)	276147..276464	-	318	WP_015063034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LJU47_RS24935 (LJU47_24935)	276483..276704	-	222	WP_015063035.1	DUF987 domain-containing protein	-
LJU47_RS24940 (LJU47_24940)	276714..277190	-	477	WP_015063036.1	RadC family protein	-
LJU47_RS24945 (LJU47_24945)	277206..277685	-	480	WP_015063037.1	antirestriction protein	-
LJU47_RS24950 (LJU47_24950)	278464..279702	+	1239	WP_015060485.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / dfrA1 / aph(3')-Ia / blaVIM-1 / qacE / sul1 / tet(A)	-	1..311760	311760

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13051.09 Da
• Isoelectric Point: 9.9961

>T221183 WP_015063033.1 NZ_CP085747:c276126-275785 [Enterobacter hormaechei]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDTGISLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQVTGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T221183 NZ_CP117356:13321-13626 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11814.47 Da
• Isoelectric Point: 6.4645

>AT221183 WP_015063034.1 NZ_CP085747:c276464-276147 [Enterobacter hormaechei]
MSNITLGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSEAECLKLDVVFPNFISQMESMLTTGEMNPRHAHCVTLYH
NDFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT221183 NZ_CP117356:13101-13319 [Salmonella enterica subsp. enterica serovar Dublin]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: J7FU23

Antitoxin

• AlphaFold DB: J7FPK5