Detailed information of TA system TA221178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 19700..20337
• Replicon: plasmid p60214CZ_IncFII
• Accession: NZ_CP085746
• Organism: Enterobacter hormaechei strain 60214CZ

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A9E6WXZ4
• Locus tag: LJU47_RS22590
• Protein ID: WP_032662169.1
• Coordinates: 19927..20337 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A9E6WTF5
• Locus tag: LJU47_RS22585
• Protein ID: WP_032662167.1
• Coordinates: 19700..19930 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU47_RS22560 (15699)	15699..16709	+	1011	WP_015572055.1	RepB family plasmid replication initiator protein	-
LJU47_RS22565 (16953)	16953..17210	-	258	Protein_21	site-specific integrase	-
LJU47_RS22570 (17412)	17412..18188	-	777	WP_015572054.1	site-specific integrase	-
LJU47_RS22575 (18207)	18207..18737	-	531	WP_013087127.1	hypothetical protein	-
LJU47_RS22580 (18868)	18868..19158	-	291	WP_013087126.1	hypothetical protein	-
LJU47_RS22585 (19700)	19700..19930	+	231	WP_032662167.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LJU47_RS22590 (19927)	19927..20337	+	411	WP_032662169.1	PIN domain-containing protein	Toxin
LJU47_RS22595 (20411)	20411..21973	+	1563	WP_032662171.1	AAA family ATPase	-
LJU47_RS22600 (21958)	21958..22980	+	1023	WP_032662174.1	helicase UvrD	-
LJU47_RS22605 (23527)	23527..24435	+	909	WP_032662179.1	HNH endonuclease	-
LJU47_RS22610 (24621)	24621..24971	-	351	WP_032662182.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-1 / aac(6')-Ib-cr / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / aac(3)-IIa	-	1..159360	159360

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14729.03 Da
• Isoelectric Point: 5.7351

>T221178 WP_032662169.1 NZ_CP085746:19927-20337 [Enterobacter hormaechei]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 411 bp

>T221178 NZ_CP117354:c4343581-4343090 [Salmonella enterica subsp. enterica serovar Dublin]
ATGATCTCCTCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAGCGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAAGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8645.77 Da
• Isoelectric Point: 4.4692

>AT221178 WP_032662167.1 NZ_CP085746:19700-19930 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT221178 NZ_CP117354:c4343871-4343578 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCCGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCCTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA