Detailed information of TA system TA221173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3102014..3102610
• Replicon: chromosome
• Accession: NZ_CP085743
• Organism: Enterobacter hormaechei strain 60214CZ

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: LJU47_RS15045
• Protein ID: WP_023315213.1
• Coordinates: 3102308..3102610 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LJU47_RS15040
• Protein ID: WP_023315212.1
• Coordinates: 3102014..3102301 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU47_RS15035 (LJU47_15035)	3100386..3102017	+	1632	WP_003860350.1	Na/Pi cotransporter family protein	-
LJU47_RS15040 (LJU47_15040)	3102014..3102301	-	288	WP_023315212.1	putative addiction module antidote protein	Antitoxin
LJU47_RS15045 (LJU47_15045)	3102308..3102610	-	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LJU47_RS15050 (LJU47_15050)	3102808..3103680	+	873	WP_022646725.1	23S rRNA pseudouridine(2604) synthase RluF	-
LJU47_RS15055 (LJU47_15055)	3103681..3103953	-	273	WP_060580348.1	DUF3811 domain-containing protein	-
LJU47_RS15060 (LJU47_15060)	3104004..3104948	-	945	WP_022650218.1	ketopantoate/pantoate/pantothenate transporter PanS	-
LJU47_RS15065 (LJU47_15065)	3105042..3106391	-	1350	WP_003860344.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T221173 WP_023315213.1 NZ_CP085743:c3102610-3102308 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T221173 NZ_CP117354:3545208-3545426 [Salmonella enterica subsp. enterica serovar Dublin]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10171.73 Da
• Isoelectric Point: 7.0086

>AT221173 WP_023315212.1 NZ_CP085743:c3102301-3102014 [Enterobacter hormaechei]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLRLTIKPSGD

Nucleotide

• Length: 288 bp

>AT221173 NZ_CP117354:3544806-3545180 [Salmonella enterica subsp. enterica serovar Dublin]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A837F9W8