Detailed information of TA system TA221026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1025923..1026760
• Replicon: chromosome
• Accession: NZ_CP085638
• Organism: Escherichia coli strain fEC.1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: LJX82_RS04765
• Protein ID: WP_000227784.1
• Coordinates: 1025923..1026465 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: LJX82_RS04770
• Protein ID: WP_001297137.1
• Coordinates: 1026449..1026760 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJX82_RS04745 (1021462)	1021462..1022373	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
LJX82_RS04750 (1022541)	1022541..1023032	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
LJX82_RS04755 (1023160)	1023160..1024524	-	1365	WP_001000960.1	MFS transporter	-
LJX82_RS04760 (1024932)	1024932..1025867	+	936	WP_001368479.1	tetratricopeptide repeat protein	-
LJX82_RS04765 (1025923)	1025923..1026465	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
LJX82_RS04770 (1026449)	1026449..1026760	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
LJX82_RS04775 (1026945)	1026945..1027835	-	891	WP_000971336.1	heme o synthase	-
LJX82_RS04780 (1027847)	1027847..1028176	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
LJX82_RS04785 (1028176)	1028176..1028790	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
LJX82_RS04790 (1028780)	1028780..1030771	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
LJX82_RS04795 (1030793)	1030793..1031740	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T221026 WP_000227784.1 NZ_CP085638:c1026465-1025923 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T221026 NZ_CP117332:212900-213385 [Salmonella enterica subsp. enterica serovar Muenster]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTATCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACTTTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT221026 WP_001297137.1 NZ_CP085638:c1026760-1026449 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT221026 NZ_CP117332:212625-212912 [Salmonella enterica subsp. enterica serovar Muenster]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3