Detailed information of TA system TA220963

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42903..43157
• Replicon: plasmid unnamed2
• Accession: NZ_CP085625
• Organism: Escherichia coli strain EC71

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LJN53_RS25270
• Protein ID: WP_001312851.1
• Coordinates: 42903..43052 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	43096..43157 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJN53_RS25225 (38476)	38476..38877	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
LJN53_RS25230 (38810)	38810..39067	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
LJN53_RS25235 (39160)	39160..39468	-	309	WP_226116723.1	CPBP family intramembrane metalloprotease	-
LJN53_RS25240 (39410)	39410..39802	-	393	WP_000616804.1	histidine phosphatase family protein	-
LJN53_RS25245 (40731)	40731..41588	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
LJN53_RS25250 (41581)	41581..42063	-	483	WP_001273588.1	hypothetical protein	-
LJN53_RS25255 (42056)	42056..42103	-	48	WP_229471593.1	hypothetical protein	-
LJN53_RS25260 (42094)	42094..42345	+	252	WP_223195197.1	replication protein RepA	-
LJN53_RS25265 (42362)	42362..42619	-	258	WP_000083845.1	replication regulatory protein RepA	-
LJN53_RS25270 (42903)	42903..43052	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (43096)	43096..43157	+	62	NuclAT_1	-	Antitoxin
- (43096)	43096..43157	+	62	NuclAT_1	-	Antitoxin
- (43096)	43096..43157	+	62	NuclAT_1	-	Antitoxin
- (43096)	43096..43157	+	62	NuclAT_1	-	Antitoxin
LJN53_RS25275 (43413)	43413..43487	-	75	Protein_53	endonuclease	-
LJN53_RS25280 (43733)	43733..43945	-	213	WP_001299730.1	ANR family transcriptional regulator	-
LJN53_RS25285 (44081)	44081..44641	-	561	WP_000139315.1	fertility inhibition protein FinO	-
LJN53_RS25290 (44744)	44744..45604	-	861	WP_000704514.1	alpha/beta hydrolase	-
LJN53_RS25295 (45663)	45663..46409	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
LJN53_RS25300 (46429)	46429..47673	-	1245	Protein_58	conjugative transfer relaxase/helicase TraI	-
LJN53_RS25305 (47675)	47675..47857	-	183	Protein_59	conjugal transfer protein TraH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	dfrA17 / blaTEM-1B / tet(B) / catA1 / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sitABCD	iutA / iucD / iucC / iucB / iucA	1..82796	82796

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T220963 WP_001312851.1 NZ_CP085625:c43052-42903 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T220963 NZ_CP117317:3374439-3374657 [Salmonella enterica subsp. enterica serovar Derby]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAACTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT220963 NZ_CP085625:43096-43157 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin