Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4141552..4142386 Replicon chromosome
Accession NZ_CP085619
Organism Escherichia coli strain EC32

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1J6XFW9
Locus tag LJN50_RS20380 Protein ID WP_000854770.1
Coordinates 4141552..4141929 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1M1N885
Locus tag LJN50_RS20385 Protein ID WP_001280950.1
Coordinates 4142018..4142386 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN50_RS20355 (4137663) 4137663..4139285 - 1623 WP_001295726.1 Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase -
LJN50_RS20360 (4140076) 4140076..4140252 - 177 Protein_3980 helix-turn-helix domain-containing protein -
LJN50_RS20365 (4140619) 4140619..4140768 - 150 Protein_3981 hypothetical protein -
LJN50_RS20370 (4140874) 4140874..4141050 - 177 WP_000839286.1 DUF957 domain-containing protein -
LJN50_RS20375 (4141067) 4141067..4141555 - 489 WP_000761690.1 DUF5983 family protein -
LJN50_RS20380 (4141552) 4141552..4141929 - 378 WP_000854770.1 TA system toxin CbtA family protein Toxin
LJN50_RS20385 (4142018) 4142018..4142386 - 369 WP_001280950.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LJN50_RS20390 (4142549) 4142549..4142770 - 222 WP_001220314.1 DUF987 domain-containing protein -
LJN50_RS20395 (4142833) 4142833..4143309 - 477 WP_001186779.1 RadC family protein -
LJN50_RS20400 (4143325) 4143325..4143789 - 465 WP_000855061.1 antirestriction protein -
LJN50_RS20405 (4144131) 4144131..4144949 - 819 WP_001234712.1 DUF932 domain-containing protein -
LJN50_RS20410 (4145067) 4145067..4145262 - 196 Protein_3990 DUF905 family protein -
LJN50_RS20415 (4145333) 4145333..4147234 - 1902 Protein_3991 Ag43/Cah family autotransporter adhesin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4129906..4170115 40209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14196.37 Da        Isoelectric Point: 8.7438

>T220929 WP_000854770.1 NZ_CP085619:c4141929-4141552 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T220929 NZ_CP117305:c4084368-4084060 [Salmonella enterica subsp. enterica serovar Derby]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGGTTAGTAAATAACCCAGTTAAGCGTCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGATGGGTTCTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13737.56 Da        Isoelectric Point: 7.0268

>AT220929 WP_001280950.1 NZ_CP085619:c4142386-4142018 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Download         Length: 369 bp

>AT220929 NZ_CP117305:c4084610-4084371 [Salmonella enterica subsp. enterica serovar Derby]
ATGTCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCTGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACGGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1J6XFW9

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1N885

References