Detailed information of TA system TA220902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 3752344..3753011
• Replicon: chromosome
• Accession: NZ_CP085532
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O50411
• Locus tag: LJF87_RS17620
• Protein ID: WP_003417916.1
• Coordinates: 3752344..3752736 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8U8S7
• Locus tag: LJF87_RS17625
• Protein ID: WP_003912214.1
• Coordinates: 3752736..3753011 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF87_RS17605 (LJF87_17590)	3747714..3749552	-	1839	WP_011799348.1	1-deoxy-D-xylulose-5-phosphate synthase	-
LJF87_RS17610 (LJF87_17595)	3749549..3750538	-	990	WP_003417912.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LJF87_RS17615 (LJF87_17600)	3750538..3751590	-	1053	WP_003900678.1	polyprenyl synthetase family protein	-
LJF87_RS17620 (LJF87_17605)	3752344..3752736	-	393	WP_003417916.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJF87_RS17625 (LJF87_17610)	3752736..3753011	-	276	WP_003912214.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LJF87_RS17630 (LJF87_17615)	3753193..3754564	+	1372	Protein_3482	ISNCY family transposase	-
LJF87_RS17635 (LJF87_17620)	3754754..3757006	+	2253	WP_011799349.1	PE family protein	-
LJF87_RS17640 (LJF87_17625)	3757077..3757949	-	873	WP_003417929.1	3-hydroxyacyl-thioester dehydratase HtdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3753887..3754564	677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14316.74 Da
• Isoelectric Point: 10.7249

>T220902 WP_003417916.1 NZ_CP085532:c3752736-3752344 [Mycobacterium tuberculosis variant bovis BCG]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRHPRVSSALARAEVMRALLDKGESARKAGRRALAHLDLLRVDKRVLDLAG
GLLPFELRTLDAIHLATAQRLGVDLGRLCTYDDRMRDAAKTLGMAVIAPS

Nucleotide

• Length: 393 bp

>T220902 NZ_CP117299:c4705125-4704607 [Salmonella enterica subsp. enterica serovar Mbandaka]
GTGGACAACCTGACAATAGAGATTCTGGCTGATAACGCAGAATACAACTGGCGGCAGTTCGACTGCGGTGAAGCATCATT
AAACCTTTTTTTAACGCAGCATCTTCAGCGCCAGCATAACAACAAAATCTTGCGCGGTTATGTACTCAGAACCACTACGC
CAGAACGGCGAGTGTTGGGGTATTACACCCTGTCAGGAAGCTGCTTTGAACGCGCGTCGTTGCCTTCCAGAACCCAGCAA
AAGCGCATCCCTTACCAGAATATCCCCAGCGTTACGCTGGGCCGTTTAGCGGTCGATCTCTCATTACAGGGAAAAGGGTG
GGGAGCCATATTGGTCACGCATGCGATGAAAGTGGTGTGGTCGGCATCACTGGCCGTTGGGATTCATGGCATCTTTGTGG
AAGCGTTAAACGAAAAAGCCCAGGCCTTTTATCAGCGGCTGGGCTTTATTTCTTTATCCGGAGAAAACGAGCACGCGCTG
TTTTACCCGACAAAGTCGATTGAGCAACTTTTTGGCTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9966.35 Da
• Isoelectric Point: 4.5332

>AT220902 WP_003912214.1 NZ_CP085532:c3753011-3752736 [Mycobacterium tuberculosis variant bovis BCG]
MTSVGVRALRQRASELLRRVEAGETIEITDRGRPVALLSPLPQGGPYEQLLASGEIERATLDVVDLPEPLDLDAGVELPS
VTLARLREHER

Nucleotide

• Length: 276 bp

>AT220902 NZ_CP117299:c4705398-4705129 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGTCAGCCCTAAAAAAACAGCGTATCGATCTGCGGCTCACAGACGGCGATAAAAGCATGATTGAAGAAGCGGCCGCCAT
TACTAATCAATCAATCACGCAATTTATGTTGAATAGCGCAGCGGAGCGAGCCGCAGAAGTGCTTGAACAGCACCGTCGGG
TTATTCTTAATGAAGCATCCTGGGCGCGAGTCATGGATGCGCTTAGTCATCCTCCCACCCCCAATGAGAAATTAAAACGC
GCGGCGCAACGTCTACAAGACATGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BXS8

Antitoxin

• AlphaFold DB: A0A0E8U8S7