Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3007251..3007938 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag LJF87_RS14235 Protein ID WP_003414064.1
Coordinates 3007543..3007938 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag LJF87_RS14230 Protein ID WP_003414061.1
Coordinates 3007251..3007517 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS14205 (LJF87_14195) 3002890..3003792 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
LJF87_RS14210 (LJF87_14200) 3003861..3004613 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
LJF87_RS14215 (LJF87_14210) 3004857..3005132 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
LJF87_RS14220 (LJF87_14215) 3005129..3006751 - 1623 WP_003414057.1 class I SAM-dependent DNA methyltransferase -
LJF87_RS14225 (LJF87_14220) 3006838..3007254 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
LJF87_RS14230 (LJF87_14225) 3007251..3007517 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LJF87_RS14235 (LJF87_14230) 3007543..3007938 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
LJF87_RS14240 (LJF87_14235) 3007935..3008204 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
LJF87_RS14245 (LJF87_14240) 3008214..3009308 - 1095 WP_003414068.1 restriction endonuclease subunit S -
LJF87_RS14250 (LJF87_14245) 3009305..3009724 - 420 Protein_2811 winged helix-turn-helix domain-containing protein -
LJF87_RS14255 (LJF87_14250) 3009723..3009797 + 75 Protein_2812 hypothetical protein -
LJF87_RS14260 (LJF87_14255) 3009798..3010277 - 480 WP_003414073.1 dihydrofolate reductase -
LJF87_RS14265 (LJF87_14260) 3010348..3011148 - 801 WP_003911953.1 thymidylate synthase -
LJF87_RS14270 (LJF87_14265) 3011304..3012041 + 738 WP_011799288.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-125)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T220893 WP_003414064.1 NZ_CP085532:c3007938-3007543 [Mycobacterium tuberculosis variant bovis BCG]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T220893 NZ_CP117299:c1442756-1442511 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGAATGCCTCCGGCGCCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCGG
CGACTGCCAGGAAGAAGCGGGATTTGAGACCGGGCGCAGCGTGACGGTGAAGATTTCGGACGGGTGTATTGTGCTAATGG
CGGACGGTAACGAGGTGCAGAAGCTGTGCGAACAGCTTTACAAGGCTGAACAGGTAGTTAAGGGGATGTGGGACGTGATT
GTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT220893 WP_003414061.1 NZ_CP085532:c3007517-3007251 [Mycobacterium tuberculosis variant bovis BCG]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT220893 NZ_CP117299:1442606-1442913 [Salmonella enterica subsp. enterica serovar Mbandaka]
CAATACACCCGTCCGAAATCTTCACCGTCACGCTGCGCCCGGTCTCAAATCCCGCTTCTTCCTGGCAGTCGCCGCTGGGG
ATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGGCGCCGGAGGCATTCATATTTGCTGA
CTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTGAGCGAGTATGGGCGCGACATCATGG
CACCGCGCCTCCCTCTCCCCGGCCAGCACCGGGCGGTGATGAGCAACCGGCTGCCGGGGCCGTATTTT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TF65


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References