Detailed information of TA system TA220891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2877735..2878449
• Replicon: chromosome
• Accession: NZ_CP085532
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: LJF87_RS13475
• Protein ID: WP_003413460.1
• Coordinates: 2878009..2878449 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: LJF87_RS13470
• Protein ID: WP_003413456.1
• Coordinates: 2877735..2878022 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF87_RS13435 (LJF87_13425)	2873167..2873412	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LJF87_RS13440 (LJF87_13430)	2873409..2873813	+	405	WP_003413432.1	type II toxin-antitoxin system VapC family toxin	-
LJF87_RS13445 (LJF87_13435)	2874030..2874650	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
LJF87_RS13450 (LJF87_13440)	2874661..2875155	+	495	WP_003413444.1	DUF2617 family protein	-
LJF87_RS13455 (LJF87_13445)	2875152..2875583	+	432	WP_003413445.1	DUF4247 domain-containing protein	-
LJF87_RS13460 (LJF87_13450)	2875608..2876056	+	449	Protein_2657	DUF350 domain-containing protein	-
LJF87_RS13465 (LJF87_13455)	2876053..2877624	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
LJF87_RS13470 (LJF87_13460)	2877735..2878022	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
LJF87_RS13475 (LJF87_13465)	2878009..2878449	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJF87_RS13480 (LJF87_13470)	2878470..2879225	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
LJF87_RS13485 (LJF87_13475)	2879358..2879954	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
LJF87_RS13490 (LJF87_13480)	2879962..2880807	-	846	WP_010950749.1	acyl-CoA thioesterase II	-
LJF87_RS13495 (LJF87_13485)	2880836..2881735	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
LJF87_RS13500 (LJF87_13490)	2881863..2882537	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T220891 WP_003413460.1 NZ_CP085532:2878009-2878449 [Mycobacterium tuberculosis variant bovis BCG]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T220891 NZ_CP117299:c1434126-1433920 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATTCCCGAACTGCATCTTAAGGGCGACTGGCTGGCCGAGGCGGGATTTGAGACCGGGACGAGCGTAACCGTGAAGATCTC
GGAGGGGTGTTTGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCTGGTGAAAAAGTCGA
TGAAGCATATTAAGGCGGGGGTAAATAATGTGGTGAACAGGGATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT220891 WP_003413456.1 NZ_CP085532:2877735-2878022 [Mycobacterium tuberculosis variant bovis BCG]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT220891 NZ_CP117299:1433930-1434115 [Salmonella enterica subsp. enterica serovar Mbandaka]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCAGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCCGAGATCTTCACGGTTACGCTCGTCCCGGTCTCAAATCCCGCCT
CGGCCAGCCAGTCGCCCTTAAGATGC

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8