Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2287908..2288544 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag LJF87_RS10670 Protein ID WP_003410654.1
Coordinates 2288134..2288544 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag LJF87_RS10665 Protein ID WP_003410651.1
Coordinates 2287908..2288141 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS10650 (LJF87_10650) 2283365..2283757 + 393 WP_229303073.1 metal ABC transporter permease -
LJF87_RS10655 (LJF87_10655) 2283758..2284162 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
LJF87_RS10660 (LJF87_10660) 2284246..2287830 - 3585 WP_011799241.1 cobaltochelatase subunit CobN -
LJF87_RS10665 (LJF87_10665) 2287908..2288141 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
LJF87_RS10670 (LJF87_10670) 2288134..2288544 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
LJF87_RS10675 (LJF87_10675) 2288528..2289619 + 1092 WP_003900454.1 precorrin-3B synthase -
LJF87_RS10680 (LJF87_10680) 2289629..2290255 + 627 WP_003410658.1 precorrin-8X methylmutase -
LJF87_RS10685 (LJF87_10685) 2290252..2291778 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
LJF87_RS10690 (LJF87_10690) 2291724..2292947 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T220883 WP_003410654.1 NZ_CP085532:2288134-2288544 [Mycobacterium tuberculosis variant bovis BCG]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T220883 NZ_CP117298:c3745581-3745189 [Mycobacterium tuberculosis variant bovis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT220883 WP_003410651.1 NZ_CP085532:2287908-2288141 [Mycobacterium tuberculosis variant bovis BCG]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT220883 NZ_CP117298:c3745856-3745581 [Mycobacterium tuberculosis variant bovis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References