Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2179300..2180003 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name mazF Uniprot ID G0TLK7
Locus tag LJF87_RS10100 Protein ID WP_003409778.1
Coordinates 2179300..2179629 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TLK8
Locus tag LJF87_RS10105 Protein ID WP_003409780.1
Coordinates 2179626..2180003 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS10080 (LJF87_10075) 2175683..2176753 + 1071 WP_003899091.1 epoxide hydrolase EphB -
LJF87_RS10085 (LJF87_10080) 2176750..2177265 + 516 WP_003409718.1 flavin reductase family protein -
LJF87_RS10090 (LJF87_10085) 2177262..2178323 + 1062 WP_003899092.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
LJF87_RS10095 (LJF87_10090) 2178320..2179090 + 771 WP_003409775.1 SDR family oxidoreductase -
LJF87_RS10100 (LJF87_10095) 2179300..2179629 - 330 WP_003409778.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LJF87_RS10105 (LJF87_10100) 2179626..2180003 - 378 WP_003409780.1 type II toxin-antitoxin system antitoxin MazE5 Antitoxin
LJF87_RS10110 (LJF87_10105) 2180000..2180590 - 591 WP_003910477.1 SEC-C metal-binding domain-containing protein -
LJF87_RS10115 (LJF87_10110) 2180645..2182009 + 1365 WP_011799233.1 HNH endonuclease signature motif containing protein -
LJF87_RS10120 (LJF87_10115) 2182164..2182616 - 453 WP_010950636.1 lipoprotein -
LJF87_RS10125 (LJF87_10120) 2182680..2183081 + 402 WP_003409869.1 hypothetical protein -
LJF87_RS10130 (LJF87_10125) 2183074..2183256 - 183 WP_003409870.1 hypothetical protein -
LJF87_RS10135 (LJF87_10130) 2183370..2183720 - 351 WP_003409871.1 hypothetical protein -
LJF87_RS10140 (LJF87_10135) 2183731..2184633 - 903 WP_003409874.1 hypothetical protein -
LJF87_RS10145 (LJF87_10140) 2184654..2184845 - 192 WP_003409876.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-103)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11787.82 Da        Isoelectric Point: 8.5361

>T220876 WP_003409778.1 NZ_CP085532:c2179629-2179300 [Mycobacterium tuberculosis variant bovis BCG]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Download         Length: 330 bp

>T220876 NZ_CP117298:c3084709-3083825 [Mycobacterium tuberculosis variant bovis]
GTGGCCGGTCTGACCCGTGCGCTCGTTGCGCGTCACGCTCTTGGTCGGGCCGAGGCATACGACGCCGCTCTGCTCGACGT
CGCCCAGGACCACCTGCTGTACCTGCTGTCGCAGACCGTCCAGTTCGGCGATAACCGCCTGGTGTTCAAAGGCGGTACCA
GCTTACGGAAGTGCCGGCTGGGCAACGTCGGGCGGTTCTCCACTGATCTGGACTTCAGCGCGCCCGACGATGAGGTCGTG
CTGGAGGTGTGCGAGCTGATCGACGGCGCCCGTGTCGGCGGCTTCGAATTCGGCGTGCAGAGCACACGAGGCGACGGCCG
GCATTGGCAGTTGCGGGTGCGGCACACGGAATTGGGCGAGCCCCGGATCGTCGCCAGCGTCGAGTTCGCACGGCGGCCGT
TGGCTTTGCCGTCTGAGCTGCTCGCATTCATCCAGTTGCCGATCCATAAGGCTTACGGTTTTGGGCTGCCGACACTGCCG
GTCGTGGCAGAAGCGGAAGCCTGCGCAGAGAAACTCGCGCGGTACCGCCGCGTTGCGCTGGCACGGGACCTGTACGACCT
GAACCACTTCGCCTCGCGAACGATTGACGAACCGCTCGTGCGGCGGCTGTGGGTGCTCAAGGTGTGGGGTGATGTCGTCG
ATGACCGGCGCGGCACCCGGCCACTACGCGTCGAAGACGTCCTCGCCGCCCGCAGCGAGCACGACTTCCAGCCCGACTCG
ATCGGCGTGCTGACCCGTCCTGTCGCTATGGCTGCCTGGGAAGCTCGCGTTCGGAAGCGATTTGCGTTCCTCACTGACCT
CGACGCCGACGAGCAGCGGTGGGCCGCCTGCGACGAACGGCACCGCCGCGAAGTGGAGAACGCGCTGGCGGTGCTGCGGT
CCTGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13560.07 Da        Isoelectric Point: 5.1519

>AT220876 WP_003409780.1 NZ_CP085532:c2180003-2179626 [Mycobacterium tuberculosis variant bovis BCG]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Download         Length: 378 bp

>AT220876 NZ_CP117298:c3085599-3084712 [Mycobacterium tuberculosis variant bovis]
GTGGTGAGCCCAGCCGGCGCCGATCGGCGCATCCCCACGTGGGCGTCACGGGTGGTGTCCGGCCTCGCTCGCGATCGGCC
CGTAGTCGTCACGAAAGAGGATCTGACGCAACGGCTTACAGAGGCTGGGTGTGGACGCGACCCCGACTCCGCCATCCGCG
AACTGCGCCGCATTGGGTGGCTGGTCCAACTGCCGGTGAAAGGCACCTGGGCATTCATTCCCCCCGGCGAGGCCGCCATC
TCGGACCCGTATCTACCGCTGCGCTCGTGGCTGGCCCGTGACCAGAACGCGGGCTTCATGCTGGCCGGTGCATCCGCAGC
GTGGCACCTCGGATACCTGGACCGCCAACCCGACGGCCGCATCCCGATCTGGCTGCCGCCGGCCAAAAGGCTGCCCGACG
GCCTAGCATCGTATGTGTCCGTCGTGCGCATCCCCTGGAACGCGGCGGACACCGCACTGCTGGCTCCGCGTCCCGCGTTG
CTGGTCCGGCGGCGGCTCGACCTCGTCGCGTGGGCGACCGGGTTACCGGCGCTAGGACCAGAAGCATTACTTGTGCAAAT
CGCCACGCGCCCGGCCTCGTTCGGGCCGTGGGCCGACCTTGTCCCCCACCTTGACGACCTCGTCGCCGACTGCAGTGACG
AGCGTCTGGAGCGTCTACTGTCGGGCCGGCCGACATCGGCCTGGCAGCGTGCAAGCTACCTGCTGGACTCGGGCGGTGAA
CCGGCACGAGGCCAGGCGCTGCTGGCCAAGCGGCACACCGAAGTGATGCCGGTGACTCGATTCACCACCGCGCACAGCCG
CGACCGTGGCGAGAGTGTCTGGGCTCCCGAGTATCAGCTTGTCGACGAGCTCGTCGTACCGCTGCTGCGCGTGATCGGCA
AGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TLK7

Antitoxin

Source ID Structure
AlphaFold DB G0TLK8

References