Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1684741..1685357 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag LJF87_RS07950 Protein ID WP_003407593.1
Coordinates 1685040..1685357 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7U4BU54
Locus tag LJF87_RS07945 Protein ID WP_011799188.1
Coordinates 1684741..1685043 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS07935 (LJF87_07910) 1680627..1682474 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
LJF87_RS07940 (LJF87_07915) 1682475..1684727 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
LJF87_RS07945 (LJF87_07920) 1684741..1685043 + 303 WP_011799188.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
LJF87_RS07950 (LJF87_07925) 1685040..1685357 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
LJF87_RS07955 (LJF87_07930) 1685354..1686358 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
LJF87_RS07960 (LJF87_07935) 1686411..1687700 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
LJF87_RS07965 (LJF87_07940) 1687773..1688498 - 726 WP_003898898.1 class I SAM-dependent methyltransferase -
LJF87_RS07970 (LJF87_07945) 1688604..1688795 - 192 WP_003407609.1 dodecin family protein -
LJF87_RS07975 1688877..1689275 + 399 WP_003900351.1 hypothetical protein -
LJF87_RS07980 (LJF87_07955) 1689320..1690348 + 1029 WP_011799189.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T220872 WP_003407593.1 NZ_CP085532:1685040-1685357 [Mycobacterium tuberculosis variant bovis BCG]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T220872 NZ_CP117298:2891053-2891493 [Mycobacterium tuberculosis variant bovis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11199.50 Da        Isoelectric Point: 9.5564

>AT220872 WP_011799188.1 NZ_CP085532:1684741-1685043 [Mycobacterium tuberculosis variant bovis BCG]
VPFLVALSGIILGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT220872 NZ_CP117298:2890779-2891066 [Mycobacterium tuberculosis variant bovis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BU54

References