Detailed information of TA system TA220869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1391793..1392352
• Replicon: chromosome
• Accession: NZ_CP085532
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: LJF87_RS06650
• Protein ID: WP_003898789.1
• Coordinates: 1391793..1392086 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: LJF87_RS06655
• Protein ID: WP_003406322.1
• Coordinates: 1392083..1392352 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF87_RS06625 (LJF87_06575)	1387386..1387646	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
LJF87_RS06630 (LJF87_06580)	1387643..1388074	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
LJF87_RS06635 (LJF87_06585)	1388097..1389785	-	1689	WP_003910308.1	PE family protein	-
LJF87_RS06640 (LJF87_06590)	1389965..1390825	+	861	WP_003406306.1	glycine betaine ABC transporter substrate-binding protein	-
LJF87_RS06645 (LJF87_06595)	1390906..1391736	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LJF87_RS06650 (LJF87_06600)	1391793..1392086	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
LJF87_RS06655 (LJF87_06605)	1392083..1392352	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LJF87_RS06660 (LJF87_06610)	1392465..1396160	-	3696	WP_010950509.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
LJF87_RS06665 (LJF87_06615)	1396302..1397090	-	789	WP_010950510.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T220869 WP_003898789.1 NZ_CP085532:c1392086-1391793 [Mycobacterium tuberculosis variant bovis BCG]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T220869 NZ_CP117298:2828859-2829272 [Mycobacterium tuberculosis variant bovis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT220869 WP_003406322.1 NZ_CP085532:c1392352-1392083 [Mycobacterium tuberculosis variant bovis BCG]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT220869 NZ_CP117298:2829311-2829568 [Mycobacterium tuberculosis variant bovis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2