Detailed information of TA system TA220868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1387386..1388074
• Replicon: chromosome
• Accession: NZ_CP085532
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: LJF87_RS06630
• Protein ID: WP_003406304.1
• Coordinates: 1387643..1388074 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: LJF87_RS06625
• Protein ID: WP_003406302.1
• Coordinates: 1387386..1387646 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF87_RS06605 (LJF87_06555)	1382963..1383787	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
LJF87_RS06610 (LJF87_06560)	1383792..1384973	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
LJF87_RS06615 (LJF87_06565)	1385050..1386150	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
LJF87_RS06620 (LJF87_06570)	1386321..1387310	+	990	WP_003406301.1	malate dehydrogenase	-
LJF87_RS06625 (LJF87_06575)	1387386..1387646	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
LJF87_RS06630 (LJF87_06580)	1387643..1388074	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJF87_RS06635 (LJF87_06585)	1388097..1389785	-	1689	WP_003910308.1	PE family protein	-
LJF87_RS06640 (LJF87_06590)	1389965..1390825	+	861	WP_003406306.1	glycine betaine ABC transporter substrate-binding protein	-
LJF87_RS06645 (LJF87_06595)	1390906..1391736	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LJF87_RS06650 (LJF87_06600)	1391793..1392086	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
LJF87_RS06655 (LJF87_06605)	1392083..1392352	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T220868 WP_003406304.1 NZ_CP085532:1387643-1388074 [Mycobacterium tuberculosis variant bovis BCG]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T220868 NZ_CP117298:c2814735-2814316 [Mycobacterium tuberculosis variant bovis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT220868 WP_003406302.1 NZ_CP085532:1387386-1387646 [Mycobacterium tuberculosis variant bovis BCG]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT220868 NZ_CP117298:c2814956-2814732 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6