Detailed information of TA system TA220867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1242670..1243238
• Replicon: chromosome
• Accession: NZ_CP085532
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: LJF87_RS05955
• Protein ID: WP_003405865.1
• Coordinates: 1242864..1243238 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: LJF87_RS05950
• Protein ID: WP_003405863.1
• Coordinates: 1242670..1242867 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF87_RS05930 (LJF87_05895)	1238711..1239349	-	639	WP_011799158.1	lipid droplet-associated protein	-
LJF87_RS05935 (LJF87_05900)	1239439..1240446	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LJF87_RS05940 (LJF87_05905)	1240463..1241446	-	984	WP_003898731.1	hypothetical protein	-
LJF87_RS05945 (LJF87_05910)	1241509..1242582	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
LJF87_RS05950 (LJF87_05915)	1242670..1242867	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LJF87_RS05955 (LJF87_05920)	1242864..1243238	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJF87_RS05960 (LJF87_05925)	1243441..1244139	+	699	WP_010950494.1	hypothetical protein	-
LJF87_RS05965 (LJF87_05930)	1244257..1244442	+	186	WP_003901093.1	hypothetical protein	-
LJF87_RS05970 (LJF87_05935)	1244369..1244644	-	276	WP_003405867.1	hypothetical protein	-
LJF87_RS05975 (LJF87_05940)	1244887..1245210	+	324	WP_003405871.1	putative quinol monooxygenase	-
LJF87_RS05980 (LJF87_05945)	1245225..1245923	-	699	WP_031646953.1	hypothetical protein	-
LJF87_RS05985 (LJF87_05950)	1245957..1246166	+	210	WP_003911400.1	hypothetical protein	-
LJF87_RS05990 (LJF87_05955)	1246118..1246888	-	771	Protein_1180	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T220867 WP_003405865.1 NZ_CP085532:1242864-1243238 [Mycobacterium tuberculosis variant bovis BCG]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T220867 NZ_CP117298:2768359-2768784 [Mycobacterium tuberculosis variant bovis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT220867 WP_003405863.1 NZ_CP085532:1242670-1242867 [Mycobacterium tuberculosis variant bovis BCG]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT220867 NZ_CP117298:2768132-2768353 [Mycobacterium tuberculosis variant bovis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07