Detailed information of TA system TA220859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 756576..757116
• Replicon: chromosome
• Accession: NZ_CP085532
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P9WII0
• Locus tag: LJF87_RS03495
• Protein ID: WP_003403376.1
• Coordinates: 756576..756884 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: LJF87_RS03500
• Protein ID: WP_003403381.1
• Coordinates: 756871..757116 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF87_RS03470 (LJF87_03445)	751891..753396	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
LJF87_RS03475 (LJF87_03450)	753477..754487	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
LJF87_RS03480 (LJF87_03455)	754875..755258	-	384	WP_003403365.1	ribonuclease VapC6	-
LJF87_RS03485 (LJF87_03460)	755353..755508	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
LJF87_RS03490 (LJF87_03465)	755584..756300	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
LJF87_RS03495 (LJF87_03470)	756576..756884	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LJF87_RS03500 (LJF87_03475)	756871..757116	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
LJF87_RS03505 (LJF87_03480)	757226..757663	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
LJF87_RS03510 (LJF87_03485)	757660..757914	-	255	WP_003911263.1	antitoxin VapB7	-
LJF87_RS03515 (LJF87_03490)	758028..760391	+	2364	WP_003403397.1	arylsulfatase AtsD	-
LJF87_RS03520 (LJF87_03495)	760454..760780	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LJF87_RS03525 (LJF87_03500)	760692..761030	+	339	WP_003403405.1	PIN domain-containing protein	-
LJF87_RS03530 (LJF87_03505)	761027..761200	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11320.10 Da
• Isoelectric Point: 11.6554

>T220859 WP_003403376.1 NZ_CP085532:c756884-756576 [Mycobacterium tuberculosis variant bovis BCG]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Nucleotide

• Length: 309 bp

>T220859 NZ_CP117298:c2188834-2188538 [Mycobacterium tuberculosis variant bovis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT220859 WP_003403381.1 NZ_CP085532:c757116-756871 [Mycobacterium tuberculosis variant bovis BCG]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT220859 NZ_CP117298:c2189082-2188831 [Mycobacterium tuberculosis variant bovis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSE5

Antitoxin

• AlphaFold DB: G0TQE0