Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 717778..718427 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag LJF87_RS03295 Protein ID WP_003403236.1
Coordinates 718032..718427 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag LJF87_RS03290 Protein ID WP_003403235.1
Coordinates 717778..718032 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS03265 (LJF87_03240) 712903..712986 + 84 Protein_647 galactose-1-phosphate uridylyltransferase -
LJF87_RS03270 (LJF87_03245) 713005..714087 + 1083 WP_031652152.1 galactose-1-phosphate uridylyltransferase -
LJF87_RS03275 (LJF87_03250) 714084..715175 + 1092 WP_003403225.1 galactokinase -
LJF87_RS03280 (LJF87_03255) 715570..716727 + 1158 WP_003903091.1 hypothetical protein -
LJF87_RS03285 (LJF87_03260) 716738..717685 + 948 WP_003403232.1 DUF732 domain-containing protein -
LJF87_RS03290 (LJF87_03265) 717778..718032 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
LJF87_RS03295 (LJF87_03270) 718032..718427 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
LJF87_RS03300 (LJF87_03275) 718521..719261 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
LJF87_RS03305 (LJF87_03280) 719393..719653 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LJF87_RS03310 (LJF87_03285) 719650..720057 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
LJF87_RS03315 (LJF87_03290) 720129..721280 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
LJF87_RS03320 (LJF87_03295) 721373..723100 - 1728 WP_010950423.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T220856 WP_003403236.1 NZ_CP085532:718032-718427 [Mycobacterium tuberculosis variant bovis BCG]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T220856 NZ_CP117298:1946191-1946439 [Mycobacterium tuberculosis variant bovis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATACGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT220856 WP_003403235.1 NZ_CP085532:717778-718032 [Mycobacterium tuberculosis variant bovis BCG]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT220856 NZ_CP117298:1945979-1946191 [Mycobacterium tuberculosis variant bovis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References