Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 696206..696852 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag LJF87_RS03140 Protein ID WP_003403122.1
Coordinates 696206..696598 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag LJF87_RS03145 Protein ID WP_003403125.1
Coordinates 696595..696852 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS03125 (LJF87_03095) 691866..693394 + 1529 Protein_619 virulence factor Mce family protein -
LJF87_RS03130 (LJF87_03100) 693457..694599 + 1143 WP_003911253.1 virulence factor Mce family protein -
LJF87_RS03135 (LJF87_03105) 694604..696154 + 1551 WP_003403119.1 MlaD family protein -
LJF87_RS03140 (LJF87_03110) 696206..696598 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
LJF87_RS03145 (LJF87_03115) 696595..696852 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
LJF87_RS03150 (LJF87_03120) 697035..698270 - 1236 WP_003403128.1 ATP-binding protein -
LJF87_RS03155 (LJF87_03125) 698521..698934 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
LJF87_RS03160 (LJF87_03130) 698931..699167 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 -
LJF87_RS03165 (LJF87_03135) 699271..699777 - 507 WP_003900973.1 two component system sensor kinase HK1 -
LJF87_RS03170 (LJF87_03140) 699891..700421 - 531 WP_003917320.1 two component system sensor kinase HK2 -
LJF87_RS03175 (LJF87_03145) 700405..701100 - 696 WP_197044064.1 two-component system response regulator TcrA -
LJF87_RS03180 (LJF87_03150) 701223..701534 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T220852 WP_003403122.1 NZ_CP085532:c696598-696206 [Mycobacterium tuberculosis variant bovis BCG]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T220852 NZ_CP117298:c1575611-1575210 [Mycobacterium tuberculosis variant bovis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT220852 WP_003403125.1 NZ_CP085532:c696852-696595 [Mycobacterium tuberculosis variant bovis BCG]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT220852 NZ_CP117298:c1575865-1575608 [Mycobacterium tuberculosis variant bovis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References