Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 365131..365774 Replicon chromosome
Accession NZ_CP085532
Organism Mycobacterium tuberculosis variant bovis BCG strain Moreau PL

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB8
Locus tag LJF87_RS01600 Protein ID WP_003401566.1
Coordinates 365349..365774 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID O07227
Locus tag LJF87_RS01595 Protein ID WP_003401563.1
Coordinates 365131..365352 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF87_RS01570 (LJF87_01570) 360205..361008 - 804 WP_003401540.1 Stf0 family sulphotransferase -
LJF87_RS01575 (LJF87_01575) 361018..362415 - 1398 WP_003401544.1 sulfatase -
LJF87_RS01580 (LJF87_01580) 362594..364414 + 1821 WP_003401546.1 PE family protein -
LJF87_RS01585 (LJF87_01585) 364557..364784 + 228 WP_003401555.1 ribbon-helix-helix protein, CopG family -
LJF87_RS01590 (LJF87_01590) 364781..365083 + 303 WP_003401560.1 toxin-antitoxin system toxin -
LJF87_RS01595 (LJF87_01595) 365131..365352 + 222 WP_003401563.1 type II toxin-antitoxin system antitoxin VapB2 Antitoxin
LJF87_RS01600 (LJF87_01600) 365349..365774 + 426 WP_003401566.1 PIN domain nuclease Toxin
LJF87_RS01605 (LJF87_01605) 365911..366543 + 633 WP_003401571.1 TetR/AcrR family transcriptional regulator -
LJF87_RS01610 (LJF87_01610) 366540..367448 + 909 WP_003900117.1 protochlorophyllide reductase -
LJF87_RS01615 367456..368046 - 591 WP_234712434.1 hypothetical protein -
LJF87_RS01620 368039..368089 - 51 Protein_320 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-125)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-37)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15713.90 Da        Isoelectric Point: 5.6002

>T220849 WP_003401566.1 NZ_CP085532:365349-365774 [Mycobacterium tuberculosis variant bovis BCG]
VTDQRWLIDKSALVRLTDSPDMEIWSNRIERGLVHITGVTRLEVGFSAECGEIARREFREPPLSAMPVEYLTPRIEDRAL
EVQTLLADRGHHRGPSIPDLLIAATAELSGLTVLHVDKDFDAIAALTGQKTERLTHRPPSA

Download         Length: 426 bp

>T220849 NZ_CP117298:1386189-1386620 [Mycobacterium tuberculosis variant bovis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8088.31 Da        Isoelectric Point: 10.7177

>AT220849 WP_003401563.1 NZ_CP085532:365131-365352 [Mycobacterium tuberculosis variant bovis BCG]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Download         Length: 222 bp

>AT220849 NZ_CP117298:1385932-1386192 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3H87

Antitoxin

Source ID Structure
PDB 3H87
AlphaFold DB A0A829CG48

References