Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 236195..236837 Replicon chromosome
Accession NZ_CP085506
Organism Bacillus cereus strain HD2.4

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag HQG80_RS01350 Protein ID WP_000635963.1
Coordinates 236487..236837 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag HQG80_RS01345 Protein ID WP_000004570.1
Coordinates 236195..236482 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQG80_RS01320 (HQG80_001320) 231512..232474 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
HQG80_RS01325 (HQG80_001325) 232467..233039 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
HQG80_RS01330 (HQG80_001330) 233133..233492 + 360 WP_000583416.1 holo-ACP synthase -
HQG80_RS01335 (HQG80_001335) 233649..234599 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
HQG80_RS01340 (HQG80_001340) 234717..235886 + 1170 WP_000390615.1 alanine racemase -
HQG80_RS01345 (HQG80_001345) 236195..236482 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
HQG80_RS01350 (HQG80_001350) 236487..236837 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HQG80_RS01355 (HQG80_001355) 236905..239073 + 2169 WP_000426242.1 Tex family protein -
HQG80_RS01360 (HQG80_001360) 239131..239247 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
HQG80_RS01365 (HQG80_001365) 239443..239901 + 459 WP_000344231.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T220816 WP_000635963.1 NZ_CP085506:236487-236837 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T220816 NZ_CP117294:805862-806263 [Salmonella enterica subsp. enterica serovar Typhi]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCCTTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGACAG
GACTGGACGCTCTTGCGTCCTCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
AG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT220816 WP_000004570.1 NZ_CP085506:236195-236482 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT220816 NZ_CP117294:805615-805881 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References