Detailed information of TA system TA022081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 894212..894750
• Replicon: chromosome
• Accession: NC_009456
• Organism: Vibrio cholerae O395

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q9KMJ0
• Locus tag: VC0395_RS04280
• Protein ID: WP_000802136.1
• Coordinates: 894451..894750 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P58093
• Locus tag: VC0395_RS04275
• Protein ID: WP_001107719.1
• Coordinates: 894212..894454 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VC0395_RS04240	889574..890173	-	600	WP_000429495.1	hypothetical protein	-
VC0395_RS04245	890328..890594	-	267	WP_000937852.1	hypothetical protein	-
VC0395_RS04250	890950..891549	-	600	WP_000429495.1	hypothetical protein	-
VC0395_RS04255	891704..891970	-	267	WP_000937852.1	hypothetical protein	-
VC0395_RS04260	892184..892867	-	684	WP_000877436.1	hypothetical protein	-
VC0395_RS20005	893291..893383	-	93	WP_014164112.1	DUF3265 domain-containing protein	-
VC0395_RS19625	893405..893983	-	579	WP_000110120.1	hypothetical protein	-
VC0395_RS04275	894212..894454	+	243	WP_001107719.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
VC0395_RS04280	894451..894750	+	300	WP_000802136.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VC0395_RS04285	894878..895273	-	396	WP_000870857.1	DUF3465 domain-containing protein	-
VC0395_RS04290	895487..895666	-	180	WP_001883058.1	DUF645 family protein	-
VC0395_RS20020	896243..896353	-	111	WP_079857360.1	DUF3265 domain-containing protein	-
VC0395_RS04300	896363..897061	-	699	WP_001890502.1	hypothetical protein	-
VC0395_RS04305	897184..897810	-	627	WP_000365423.1	LysE family translocator	-
VC0395_RS20025	897852..897944	-	93	WP_014378730.1	DUF3265 domain-containing protein	-
VC0395_RS04310	897959..898432	-	474	WP_001161076.1	GrpB family protein	-
VC0395_RS04315	898632..898814	-	183	WP_000923182.1	DUF645 family protein	-
VC0395_RS20575	898982..899104	-	123	WP_086027501.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	833458..916350	82892

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11589.29 Da
• Isoelectric Point: 9.9232

>T22081 WP_000802136.1 NC_009456:894451-894750 [Vibrio cholerae O395]
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKFPQGSHVIFYQQTGSQQ
IRVIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T22081 NC_009456:894451-894750 [Vibrio cholerae O395]
ATGAAACCATTTAATCTTACCGTCGCCGCCAAAGCCGATTTACGTGATATTGCTTTATTCACTCAACGACGCTGGGGAAA
AGAGCAGCGAAATGTTTATTTAAAGCAATTCGATGATTCCTTTTGGCTTTTAGCGGAAAATCCCGACATTGGTAAATCAT
GCGATGAAATCCGAGAGGGATACAGAAAATTTCCCCAAGGGAGTCACGTCATCTTTTATCAGCAAACCGGCAGCCAACAA
ATCAGGGTGATCCGAATTCTTCATAAGAGCATGGATGTGAACCCAATATTCGGCGCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8964.84 Da
• Isoelectric Point: 4.1677

>AT22081 WP_001107719.1 NC_009456:894212-894454 [Vibrio cholerae O395]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLIEGEQSGDADYDLDSFINELDSENIR

Nucleotide

• Length: 243 bp

>AT22081 NC_009456:894212-894454 [Vibrio cholerae O395]
ATGGCTAAAAATACAAGTATCACTCTTGGTGAACACTTCGATGGCTTTATTACAAGCCAAATACAAAGTGGGCGTTACGG
CTCAGCAAGTGAAGTCATTCGCTCTGCGCTACGTCTACTCGAAAACCAAGAAACCAAACTACAGTCACTCCGTCAACTAC
TTATTGAAGGAGAGCAAAGTGGTGACGCTGATTATGACCTTGATAGCTTCATCAATGAACTCGATAGTGAAAACATTCGA
TGA

Structures

Toxin

• PDB: 7R5A

Antitoxin

• PDB: 7R5A
• PDB: 7B22
• AlphaFold DB: A0A151JFU4