Detailed information of TA system TA220719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1216054..1216651
• Replicon: chromosome
• Accession: NZ_CP085326
• Organism: Bifidobacterium pseudolongum strain 46-D-6

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: LJD78_RS04925
• Protein ID: WP_080699166.1
• Coordinates: 1216054..1216356 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N3QV32
• Locus tag: LJD78_RS04930
• Protein ID: WP_026643099.1
• Coordinates: 1216358..1216651 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJD78_RS04895 (LJD78_04895)	1211890..1212708	+	819	WP_227334615.1	dihydroorotate dehydrogenase electron transfer subunit	-
LJD78_RS04900 (LJD78_04900)	1212693..1213682	+	990	WP_129989983.1	dihydroorotate dehydrogenase	-
LJD78_RS04905 (LJD78_04905)	1213723..1214418	+	696	WP_039173251.1	orotate phosphoribosyltransferase	-
LJD78_RS04910 (LJD78_04910)	1214452..1215270	+	819	WP_080699168.1	serpin family protein	-
LJD78_RS04915 (LJD78_04915)	1215506..1215742	+	237	WP_225531405.1	hypothetical protein	-
LJD78_RS04920 (LJD78_04920)	1215755..1215943	+	189	WP_225532136.1	GNAT family N-acetyltransferase	-
LJD78_RS04925 (LJD78_04925)	1216054..1216356	+	303	WP_080699166.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LJD78_RS04930 (LJD78_04930)	1216358..1216651	+	294	WP_026643099.1	putative addiction module antidote protein	Antitoxin
LJD78_RS04935 (LJD78_04935)	1216773..1217117	-	345	WP_225531406.1	helix-turn-helix domain-containing protein	-
LJD78_RS04940 (LJD78_04940)	1217284..1218213	-	930	WP_026643097.1	LysR family transcriptional regulator	-
LJD78_RS04945 (LJD78_04945)	1218247..1218888	-	642	WP_225531407.1	HAD family phosphatase	-
LJD78_RS04950 (LJD78_04950)	1219083..1220831	-	1749	WP_225531408.1	FAD-dependent oxidoreductase	-
LJD78_RS04955 (LJD78_04955)	1220858..1221421	-	564	WP_033488853.1	alkyl hydroperoxide reductase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-95)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11494.14 Da
• Isoelectric Point: 9.8170

>T220719 WP_080699166.1 NZ_CP085326:1216054-1216356 [Bifidobacterium pseudolongum]
MEIRTTEQFDKWFRKLRDRKAKAKISARLRQCEISDHITGDHHGIGGGISEMRFHFGPGYRIYYTVDNDVLVILLIGSGK
DDQSRGIAEARKLLALYRGE

Nucleotide

• Length: 303 bp

>T220719 NZ_CP117256:148944-149258 [Rhizobium tumorigenes]
ATGCAACTTGAGACATTTGAGTACGACTCCGCCGAATTTCTTGGAAGTGAGGAAGCGATTGCTGAATATCTTTTAGCTGC
TAGCGAAGATGGAGACGCAAACCATATTGCTCGCGCTCTTGGTGTTGTCGCGCGGGCGCGCGGCATGTCCGATCTTTCTC
GGAATACCGGACTTACGCGACCTGCTCTTTACCGGGCAATAAGTGGAGATGGCAATCCAGAGTTTGGGACAATCGCAAAG
GTAGCATACGCACTTGGTTATAGGCTAAATCTCGTTTTGAAATCCGACAGCGGATCGACCGCCGCGGTGAAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10542.01 Da
• Isoelectric Point: 4.5837

>AT220719 WP_026643099.1 NZ_CP085326:1216358-1216651 [Bifidobacterium pseudolongum]
MTLKTTKWDVTEYLDNEEDIAEYLNAVIELDDPALLQAAIGDVAKAQGMTKIAKDAGLGRESLYKSLSAQGNPSFTTILK
VLHALGTRISIQPEHAI

Nucleotide

• Length: 294 bp

>AT220719 NZ_CP117256:148643-148906 [Rhizobium tumorigenes]
ATGTTCACGATTAGGCGCACCCGTAATTATGCGGACTGGATCAAAACGCTAAAGGACTTTCGTGCCCAAGCTCGAATAGC
CGACCGCATTGACCGTCTCGCCGCAGGAAATCCTGGGGATGTAAAGCCAGTCGGCGAAGGCGTGAGCGAACTCCGCATTA
ACTACGGGCCTGGTTATCGTGTCAATTTTGTCCGGGAAGGGTTGGTCGTCTATGTTCTCCTCTGTCGTGGCGACAAGAGT
ACCCAAGAGAAAGATATTCGATAG

Structures

Antitoxin

• AlphaFold DB: A0A2N3QV32