Detailed information of TA system TA220558

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30811..31080
• Replicon: plasmid pEC7518-49k
• Accession: NZ_CP085186
• Organism: Escherichia coli strain EC7518

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LIN51_RS25195
• Protein ID: WP_001372321.1
• Coordinates: 30955..31080 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30811..30876 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIN51_RS25160	26521..27048	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LIN51_RS25165	27106..27339	+	234	WP_000006003.1	DUF905 family protein	-
LIN51_RS25170	27400..29423	+	2024	Protein_38	ParB/RepB/Spo0J family partition protein	-
LIN51_RS25175	29492..29926	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LIN51_RS25180	29923..30685	+	763	Protein_40	plasmid SOS inhibition protein A	-
-	30654..30878	+	225	NuclAT_0	-	-
-	30654..30878	+	225	NuclAT_0	-	-
-	30654..30878	+	225	NuclAT_0	-	-
-	30654..30878	+	225	NuclAT_0	-	-
LIN51_RS25185	30663..30842	-	180	WP_001309233.1	hypothetical protein	-
-	30811..30876	-	66	-	-	Antitoxin
LIN51_RS25190	30864..31013	+	150	Protein_42	plasmid maintenance protein Mok	-
LIN51_RS25195	30955..31080	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LIN51_RS25200	31399..31695	-	297	Protein_44	hypothetical protein	-
LIN51_RS25205	31995..32291	+	297	WP_001272251.1	hypothetical protein	-
LIN51_RS25210	32402..33223	+	822	WP_001234445.1	DUF932 domain-containing protein	-
LIN51_RS25215	33520..34167	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
LIN51_RS25220	34444..34827	+	384	WP_227311784.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LIN51_RS25225	35018..35704	+	687	WP_015059009.1	PAS domain-containing protein	-
LIN51_RS25230	35798..36025	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaTEM-1B / rmtB	-	1..49706	49706

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T220558 WP_001372321.1 NZ_CP085186:30955-31080 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T220558 NZ_CP117181:887899-889224 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT220558 NZ_CP085186:c30876-30811 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin