Detailed information of TA system TA022017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 57118..57652
• Replicon: plasmid CD
• Accession: NC_009377
• Organism: Yersinia pestis Pestoides F

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: YPDSF_RS21210
• Protein ID: WP_002213360.1
• Coordinates: 57353..57652 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: YPDSF_RS21205
• Protein ID: WP_002224342.1
• Coordinates: 57118..57360 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YPDSF_RS21195	53817..54983	+	1167	WP_002220902.1	plasmid-partitioning protein SopA	-
YPDSF_RS21200	54980..55945	+	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
YPDSF_RS24540	56400..56573	-	174	WP_002213357.1	hypothetical protein	-
YPDSF_RS23850	56660..56952	+	293	Protein_71	IS6 family transposase	-
YPDSF_RS21205	57118..57360	+	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
YPDSF_RS21210	57353..57652	+	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
YPDSF_RS21215	57792..58451	-	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
YPDSF_RS21220	58645..59037	+	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
YPDSF_RS21225	59100..59744	-	645	Protein_76	IS3 family transposase	-
YPDSF_RS21235	59847..61019	+	1173	WP_002220893.1	IS21 family transposase	-
YPDSF_RS21240	61019..61417	+	399	Protein_78	ATP-binding protein	-
YPDSF_RS21245	61794..62219	+	426	WP_002213267.1	type III secretion system chaperone SycH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yopO/ypkA / yopJ/yopP / yopH / lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscQ / yscP / yscO / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG / lcrV / sycD/lcrH / yopB / yopD / yopM / sycT / yopT / yopK/yopQ / yopE / sycE/yerA / sycH / yadA / yadA	1..71507	71507

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T22017 WP_002213360.1 NC_009377:57353-57652 [Yersinia pestis Pestoides F]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T22017 NC_009377:57353-57652 [Yersinia pestis Pestoides F]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT22017 WP_002224342.1 NC_009377:57118-57360 [Yersinia pestis Pestoides F]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT22017 NC_009377:57118-57360 [Yersinia pestis Pestoides F]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6