Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3791313..3791903 Replicon chromosome
Accession NZ_CP084868
Organism Klebsiella pneumoniae strain SA1

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag LGM08_RS18225 Protein ID WP_022615588.1
Coordinates 3791313..3791645 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag LGM08_RS18230 Protein ID WP_000288812.1
Coordinates 3791646..3791903 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM08_RS18195 (3787360) 3787360..3787605 + 246 WP_004141189.1 signal transduction protein PmrD -
LGM08_RS18200 (3787735) 3787735..3789189 + 1455 WP_004148975.1 AMP nucleosidase -
LGM08_RS18210 (3789555) 3789555..3790991 - 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
LGM08_RS18225 (3791313) 3791313..3791645 - 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LGM08_RS18230 (3791646) 3791646..3791903 - 258 WP_000288812.1 antitoxin Antitoxin
LGM08_RS18235 (3792238) 3792238..3792444 - 207 WP_022615589.1 helix-turn-helix transcriptional regulator -
LGM08_RS18240 (3792441) 3792441..3792902 - 462 WP_004213450.1 hypothetical protein -
LGM08_RS18245 (3793185) 3793185..3793741 - 557 Protein_3556 hypothetical protein -
LGM08_RS18250 (3793820) 3793820..3795568 - 1749 WP_032457402.1 hypothetical protein -
LGM08_RS18255 (3795918) 3795918..3795995 - 78 Protein_3558 type II toxin-antitoxin system PemK/MazF family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T219668 WP_022615588.1 NZ_CP084868:c3791645-3791313 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T219668 NZ_CP116571:c2822135-2821794 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT219668 WP_000288812.1 NZ_CP084868:c3791903-3791646 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT219668 NZ_CP116571:c2822365-2822135 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References