Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 13676..14201 Replicon plasmid pKp_SB61067_1
Accession NZ_CP084861
Organism Klebsiella pneumoniae strain SB1067

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag LGM10_RS25645 Protein ID WP_013023785.1
Coordinates 13676..13981 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag LGM10_RS25650 Protein ID WP_001568025.1
Coordinates 13983..14201 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM10_RS25610 (LGM10_25610) 8838..9806 + 969 WP_226616199.1 IS5 family transposase -
LGM10_RS25615 (LGM10_25615) 10338..10451 + 114 WP_014343462.1 hypothetical protein -
LGM10_RS25620 (LGM10_25620) 10580..10837 + 258 WP_009310077.1 hypothetical protein -
LGM10_RS25625 (LGM10_25625) 10895..11674 - 780 WP_023287113.1 site-specific integrase -
LGM10_RS25630 (LGM10_25630) 11872..12888 - 1017 WP_009309921.1 hypothetical protein -
LGM10_RS25635 (LGM10_25635) 12922..13257 - 336 WP_009309920.1 hypothetical protein -
LGM10_RS25640 (LGM10_25640) 13307..13447 - 141 WP_162898808.1 hypothetical protein -
LGM10_RS25645 (LGM10_25645) 13676..13981 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
LGM10_RS25650 (LGM10_25650) 13983..14201 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LGM10_RS25655 (LGM10_25655) 14754..15677 + 924 WP_025714719.1 hypothetical protein -
LGM10_RS25660 (LGM10_25660) 15721..16011 + 291 WP_023287115.1 hypothetical protein -
LGM10_RS25665 (LGM10_25665) 16497..16841 + 345 WP_009483875.1 hypothetical protein -
LGM10_RS25670 (LGM10_25670) 17026..17442 - 417 Protein_21 DUF4238 domain-containing protein -
LGM10_RS25675 (LGM10_25675) 17522..18426 + 905 Protein_22 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..226445 226445


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T219631 WP_013023785.1 NZ_CP084861:c13981-13676 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T219631 NZ_CP116564:482413-482529 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGCATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT219631 WP_001568025.1 NZ_CP084861:c14201-13983 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT219631 NZ_CP116564:c482645-482440 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATGC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References