Detailed information of TA system TA219473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 78919..79670
• Replicon: plasmid pKp_T69_1
• Accession: NZ_CP084824
• Organism: Klebsiella pneumoniae strain T69

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: LGM19_RS26390
• Protein ID: WP_004902249.1
• Coordinates: 79188..79670 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: LGM19_RS26385
• Protein ID: WP_004902250.1
• Coordinates: 78919..79197 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM19_RS26350 (LGM19_26350)	73947..74237	+	291	WP_011154627.1	MSMEG_0570 family nitrogen starvation response protein	-
LGM19_RS26355 (LGM19_26355)	74255..75520	+	1266	WP_004210292.1	MSMEG_0569 family flavin-dependent oxidoreductase	-
LGM19_RS26360 (LGM19_26360)	75501..77171	+	1671	WP_004902261.1	AMP-binding protein	-
LGM19_RS26365 (LGM19_26365)	77255..77365	-	111	Protein_86	glutathione ABC transporter permease GsiC	-
LGM19_RS26370 (LGM19_26370)	77409..77678	+	270	WP_071591895.1	hypothetical protein	-
LGM19_RS26375 (LGM19_26375)	78139..78480	+	342	WP_004902257.1	hypothetical protein	-
LGM19_RS26380 (LGM19_26380)	78588..78800	+	213	WP_004902255.1	hypothetical protein	-
LGM19_RS26385 (LGM19_26385)	78919..79197	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
LGM19_RS26390 (LGM19_26390)	79188..79670	+	483	WP_004902249.1	GNAT family N-acetyltransferase	Toxin
LGM19_RS26395 (LGM19_26395)	80749..81446	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
LGM19_RS26400 (LGM19_26400)	82832..83524	-	693	WP_011154578.1	DUF1173 family protein	-
LGM19_RS26405 (LGM19_26405)	83515..83781	-	267	WP_223175074.1	DUF1173 family protein	-
LGM19_RS26410 (LGM19_26410)	83960..84454	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..206626	206626
	-	flank	IS/Tn	-	-	80943..81446	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17689.52 Da
• Isoelectric Point: 9.2033

>T219473 WP_004902249.1 NZ_CP084824:79188-79670 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNKVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T219473 NZ_CP116477:c913760-913455 [Pectobacterium sp. A5351]
ATGCAATTCATTGTTTATCAACACAAAATAACCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTTGAAAC
ACCAAAACGGCGCATGGTCATCCCGCTTATCGAATCACATCACCTGTCTGAGAAAGTGAATAAGACTTTGTTCCCTCTGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACCACTGAGCTATCGAGCGTTCCTGTTGAGGTTATTGGTGAAGTGATT
GCGGATCTTGGCGACTACGCGGATGAGATCAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT219473 WP_004902250.1 NZ_CP084824:78919-79197 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT219473 NZ_CP116477:c913981-913763 [Pectobacterium sp. A5351]
ATGAAACACCGCGTAAGCGTTACCGTAGACAAAGATAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCTGGGCT
GGTGAATGATGCCATTGGCAAAGAAGCCCGCCGCATCAGGGCTGAAGAGTGGAAAAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCAATTTATCGCACAGAATGGCTCGTTTGCGGATGAAAACAGGAACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3