Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2580801..2582389 Replicon chromosome
Accession NZ_CP084783
Organism Klebsiella quasipneumoniae subsp. similipneumoniae strain 09A323

Toxin (Protein)


Gene name hipA Uniprot ID A0A246AZS4
Locus tag LGM26_RS12500 Protein ID WP_017900381.1
Coordinates 2581067..2582389 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A2A5MFC9
Locus tag LGM26_RS12495 Protein ID WP_004205921.1
Coordinates 2580801..2581067 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM26_RS12470 (2575934) 2575934..2576857 + 924 WP_008804925.1 LysR family hpxDE operon transcriptional regulator HpxR -
LGM26_RS12475 (2576838) 2576838..2577992 - 1155 WP_136070648.1 FAD-dependent urate hydroxylase HpxO -
LGM26_RS12480 (2578338) 2578338..2579717 + 1380 WP_017900377.1 nucleobase:cation symporter-2 family protein -
LGM26_RS12485 (2579695) 2579695..2580195 + 501 WP_017900378.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
LGM26_RS12490 (2580192) 2580192..2580518 + 327 WP_017900379.1 hydroxyisourate hydrolase -
LGM26_RS12495 (2580801) 2580801..2581067 + 267 WP_004205921.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
LGM26_RS12500 (2581067) 2581067..2582389 + 1323 WP_017900381.1 type II toxin-antitoxin system HipA family toxin Toxin
LGM26_RS12505 (2582406) 2582406..2583698 - 1293 WP_017900382.1 glycoside hydrolase family 10 protein -
LGM26_RS12510 (2583840) 2583840..2584046 - 207 WP_004205917.1 helix-turn-helix transcriptional regulator -
LGM26_RS12515 (2584049) 2584049..2584582 - 534 WP_004205916.1 hypothetical protein -
LGM26_RS12520 (2584611) 2584611..2584955 - 345 WP_017900383.1 hypothetical protein -
LGM26_RS12525 (2584942) 2584942..2586057 - 1116 WP_017900384.1 serine hydrolase domain-containing protein -
LGM26_RS12530 (2586326) 2586326..2587108 - 783 WP_017900385.1 type I methionyl aminopeptidase -
LGM26_RS12535 (2587089) 2587089..2587307 - 219 WP_002904808.1 ParD-like family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49041.33 Da        Isoelectric Point: 8.6091

>T219394 WP_017900381.1 NZ_CP084783:2581067-2582389 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILGEFADRVP
QAIESARRTLPSDFSAHVWRAITENMLKLHARLQQGLQAG

Download         Length: 1323 bp

>T219394 NZ_CP116405:886874-887281 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9940.38 Da        Isoelectric Point: 7.1997

>AT219394 WP_004205921.1 NZ_CP084783:2580801-2581067 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Download         Length: 267 bp

>AT219394 NZ_CP116405:886627-886893 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A246AZS4

Antitoxin

Source ID Structure
AlphaFold DB A0A2A5MFC9

References