Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5462813..5463438 Replicon chromosome
Accession NZ_CP084767
Organism Klebsiella variicola subsp. tropica strain CDC4241-71

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LGN97_RS26405 Protein ID WP_002882817.1
Coordinates 5462813..5463196 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LGN97_RS26410 Protein ID WP_004150355.1
Coordinates 5463196..5463438 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGN97_RS26390 (LGN97_26390) 5460179..5461081 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LGN97_RS26395 (LGN97_26395) 5461078..5461713 + 636 WP_061156261.1 formate dehydrogenase cytochrome b556 subunit -
LGN97_RS26400 (LGN97_26400) 5461710..5462639 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LGN97_RS26405 (LGN97_26405) 5462813..5463196 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LGN97_RS26410 (LGN97_26410) 5463196..5463438 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LGN97_RS26415 (LGN97_26415) 5463643..5464560 + 918 WP_074387461.1 alpha/beta hydrolase -
LGN97_RS26420 (LGN97_26420) 5464575..5465516 - 942 WP_165784254.1 fatty acid biosynthesis protein FabY -
LGN97_RS26425 (LGN97_26425) 5465561..5465998 - 438 WP_012543288.1 D-aminoacyl-tRNA deacylase -
LGN97_RS26430 (LGN97_26430) 5465995..5466855 - 861 WP_074387464.1 virulence factor BrkB family protein -
LGN97_RS26435 (LGN97_26435) 5466849..5467448 - 600 WP_074387465.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T219344 WP_002882817.1 NZ_CP084767:c5463196-5462813 [Klebsiella variicola subsp. tropica]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T219344 NZ_CP116313:c3485549-3484812 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCAAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCTGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCACAATTTAGTATTATCGTCCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT219344 WP_004150355.1 NZ_CP084767:c5463438-5463196 [Klebsiella variicola subsp. tropica]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT219344 NZ_CP116313:c3484699-3484571 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References