Detailed information of TA system TA219225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 738383..738907
• Replicon: chromosome
• Accession: NZ_CP084719
• Organism: Staphylococcus chromogenes strain 76ME

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LGV34_RS03495
• Protein ID: WP_103159488.1
• Coordinates: 738554..738907 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A3A0K2V6
• Locus tag: LGV34_RS03490
• Protein ID: WP_037577177.1
• Coordinates: 738383..738553 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGV34_RS03465 (LGV34_03465)	734231..734704	+	474	WP_051605022.1	PH domain-containing protein	-
LGV34_RS03470 (LGV34_03470)	734697..736175	+	1479	WP_268217046.1	PH domain-containing protein	-
LGV34_RS03475 (LGV34_03475)	736172..736687	+	516	WP_051605021.1	PH domain-containing protein	-
LGV34_RS03480 (LGV34_03480)	736684..737034	+	351	WP_037577181.1	holo-ACP synthase	-
LGV34_RS03485 (LGV34_03485)	737150..738298	+	1149	WP_268217047.1	alanine racemase	-
LGV34_RS03490 (LGV34_03490)	738383..738553	+	171	WP_037577177.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LGV34_RS03495 (LGV34_03495)	738554..738907	+	354	WP_103159488.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LGV34_RS03500 (LGV34_03500)	738971..739975	+	1005	WP_268217048.1	PP2C family protein-serine/threonine phosphatase	-
LGV34_RS03505 (LGV34_03505)	740056..740382	+	327	WP_037577172.1	anti-sigma factor antagonist	-
LGV34_RS03510 (LGV34_03510)	740385..740864	+	480	WP_037577169.1	anti-sigma B factor RsbW	-
LGV34_RS03515 (LGV34_03515)	740839..741609	+	771	WP_037577167.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13064.34 Da
• Isoelectric Point: 10.5127

>T219225 WP_103159488.1 NZ_CP084719:738554-738907 [Staphylococcus chromogenes]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIDKKKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSDKKMKEVNAALGISLGLHMNQHK

Nucleotide

• Length: 354 bp

>T219225 NZ_CP116214:c5246407-5246120 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTATCGGTC
TCGTTCATGGGGTCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6328.01 Da
• Isoelectric Point: 4.2313

>AT219225 WP_037577177.1 NZ_CP084719:738383-738553 [Staphylococcus chromogenes]
MSAFNQTRVKSLEQSLKEGYVQMADLNLSLATEAYFVECEACDCNESHLLSNHKDD

Nucleotide

• Length: 171 bp

>AT219225 NZ_CP116214:c5246133-5245831 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGGTATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACTCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A3A0K2V6