Detailed information of TA system TA219209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 6724..7304
• Replicon: plasmid p4
• Accession: NZ_CP084709
• Organism: Klebsiella pneumoniae strain KP1064WHY

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: K8U55_RS30005
• Protein ID: WP_071177730.1
• Coordinates: 6990..7304 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: K8U55_RS30000
• Protein ID: WP_000093040.1
• Coordinates: 6724..7002 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8U55_RS29980 (K8U55_29975)	3165..4652	-	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
K8U55_RS29985 (K8U55_29980)	5297..5542	+	246	WP_032440458.1	hypothetical protein	-
K8U55_RS29990 (K8U55_29985)	5816..6187	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
K8U55_RS29995 (K8U55_29990)	6184..6549	+	366	WP_072354022.1	TonB family protein	-
K8U55_RS30000 (K8U55_29995)	6724..7002	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
K8U55_RS30005 (K8U55_30000)	6990..7304	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8U55_RS30010 (K8U55_30005)	7468..7896	+	429	WP_001140599.1	hypothetical protein	-
K8U55_RS30015 (K8U55_30010)	7922..8101	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
K8U55_RS30020 (K8U55_30015)	8128..8658	-	531	WP_226522486.1	hypothetical protein	-
K8U55_RS30025 (K8U55_30020)	8665..9300	-	636	WP_226522487.1	replication-relaxation family protein	-
K8U55_RS30030 (K8U55_30025)	9395..11359	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11972	11972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T219209 WP_071177730.1 NZ_CP084709:6990-7304 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T219209 NZ_CP116197:c4689969-4689658 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT219209 WP_000093040.1 NZ_CP084709:6724-7002 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT219209 NZ_CP116197:c4689657-4689367 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1