Detailed information of TA system TA021891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3024032..3024719
• Replicon: chromosome
• Accession: NC_008769
• Organism: Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: BCG_RS14295
• Protein ID: WP_003414064.1
• Coordinates: 3024324..3024719 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: BCG_RS14290
• Protein ID: WP_003414061.1
• Coordinates: 3024032..3024298 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCG_RS14265	3019671..3020573	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BCG_RS14270	3020642..3021394	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
BCG_RS14275	3021638..3021913	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
BCG_RS14280	3021910..3023532	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
BCG_RS14285	3023619..3024035	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
BCG_RS14290	3024032..3024298	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BCG_RS14295	3024324..3024719	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCG_RS14300	3024716..3024985	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
BCG_RS14305	3024995..3026089	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
BCG_RS14310	3026086..3026505	-	420	Protein_2793	winged helix-turn-helix domain-containing protein	-
BCG_RS14315	3026579..3027058	-	480	WP_003414073.1	dihydrofolate reductase	-
BCG_RS14320	3027129..3027929	-	801	WP_003911953.1	thymidylate synthase	-
BCG_RS14325	3028085..3028822	+	738	WP_011799288.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T21891 WP_003414064.1 NC_008769:c3024719-3024324 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T21891 NC_008769:c3024719-3024324 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT21891 WP_003414061.1 NC_008769:c3024298-3024032 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT21891 NC_008769:c3024298-3024032 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64