Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2834179..2834816 Replicon chromosome
Accession NC_008769
Organism Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag BCG_RS13295 Protein ID WP_003413180.1
Coordinates 2834179..2834574 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag BCG_RS13300 Protein ID WP_003413183.1
Coordinates 2834571..2834816 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCG_RS13255 2830264..2830923 + 660 WP_003900846.1 LppA family lipoprotein -
BCG_RS13260 2830920..2831579 + 660 WP_003900847.1 hypothetical protein -
BCG_RS13265 2831576..2832238 + 663 WP_003900848.1 hypothetical protein -
BCG_RS13270 2832235..2832513 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
BCG_RS13275 2832606..2833019 + 414 WP_003413164.1 PIN domain nuclease -
BCG_RS13280 2833058..2833315 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
BCG_RS13285 2833312..2833689 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
BCG_RS13290 2833705..2834079 - 375 WP_003413177.1 hypothetical protein -
BCG_RS13295 2834179..2834574 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
BCG_RS13300 2834571..2834816 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
BCG_RS13305 2835227..2835646 - 420 WP_003413190.1 A24 family peptidase -
BCG_RS13310 2835658..2836485 - 828 WP_010950736.1 shikimate dehydrogenase -
BCG_RS13315 2836463..2837716 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BCG_RS13320 2837709..2838221 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T21888 WP_003413180.1 NC_008769:c2834574-2834179 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T21888 NC_008769:c2834574-2834179 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT21888 WP_003413183.1 NC_008769:c2834816-2834571 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT21888 NC_008769:c2834816-2834571 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References