Detailed information of TA system TA021874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2196804..2197507
• Replicon: chromosome
• Accession: NC_008769
• Organism: Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BCG_RS10180
• Protein ID: WP_003409778.1
• Coordinates: 2196804..2197133 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BCG_RS21070
• Protein ID: WP_003409780.1
• Coordinates: 2197130..2197507 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCG_RS10160	2193187..2194257	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
BCG_RS10165	2194254..2194769	+	516	WP_003409718.1	flavin reductase family protein	-
BCG_RS10170	2194766..2195827	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BCG_RS10175	2195824..2196594	+	771	WP_003409775.1	SDR family oxidoreductase	-
BCG_RS10180	2196804..2197133	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BCG_RS21070	2197130..2197507	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BCG_RS10190	2197504..2198094	-	591	WP_003910477.1	SEC-C domain-containing protein	-
BCG_RS10195	2198149..2199513	+	1365	WP_011799233.1	HNH endonuclease	-
BCG_RS10200	2199668..2200120	-	453	WP_010950636.1	lipoprotein	-
BCG_RS10205	2200184..2200585	+	402	WP_003409869.1	hypothetical protein	-
BCG_RS10210	2200578..2200760	-	183	WP_003409870.1	hypothetical protein	-
BCG_RS10215	2200874..2201224	-	351	WP_003409871.1	hypothetical protein	-
BCG_RS10220	2201235..2202137	-	903	WP_003409874.1	hypothetical protein	-
BCG_RS10225	2202158..2202349	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T21874 WP_003409778.1 NC_008769:c2197133-2196804 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T21874 NC_008769:c2197133-2196804 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT21874 WP_003409780.1 NC_008769:c2197507-2197130 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT21874 NC_008769:c2197507-2197130 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8