Detailed information of TA system TA218713

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30982..31251
• Replicon: plasmid p1632-1
• Accession: NZ_CP084498
• Organism: Klebsiella pneumoniae strain 1632

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LDL62_RS27375
• Protein ID: WP_001372321.1
• Coordinates: 31126..31251 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30982..31047 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDL62_RS27345	26692..27219	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LDL62_RS27350	27277..27510	+	234	WP_000006003.1	DUF905 family protein	-
LDL62_RS27355	27571..29594	+	2024	Protein_37	ParB/RepB/Spo0J family partition protein	-
LDL62_RS27360	29663..30097	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LDL62_RS27365	30094..30813	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	30825..31049	+	225	NuclAT_0	-	-
-	30825..31049	+	225	NuclAT_0	-	-
-	30825..31049	+	225	NuclAT_0	-	-
-	30825..31049	+	225	NuclAT_0	-	-
-	30982..31047	-	66	-	-	Antitoxin
LDL62_RS27370	31035..31184	+	150	Protein_40	plasmid maintenance protein Mok	-
LDL62_RS27375	31126..31251	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LDL62_RS27380	31570..31866	-	297	Protein_42	hypothetical protein	-
LDL62_RS27385	32166..32462	+	297	WP_001272251.1	hypothetical protein	-
LDL62_RS27390	32573..33394	+	822	WP_001234445.1	DUF932 domain-containing protein	-
LDL62_RS27395	33691..34338	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
LDL62_RS27400	34615..34998	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LDL62_RS27405	35189..35875	+	687	WP_015059009.1	PAS domain-containing protein	-
LDL62_RS27410	35969..36196	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..163933	163933

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T218713 WP_001372321.1 NZ_CP084498:31126-31251 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T218713 NZ_CP116085:c3854271-3853351 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT218713 NZ_CP084498:c31047-30982 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin