Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 151629..151882 Replicon plasmid p1864-2
Accession NZ_CP084494
Organism Klebsiella pneumoniae strain 1864

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag LDL64_RS29255 Protein ID WP_001312851.1
Coordinates 151733..151882 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag -
Coordinates 151629..151688 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDL64_RS29215 (147289) 147289..147354 - 66 Protein_197 helix-turn-helix domain-containing protein -
LDL64_RS29220 (147407) 147407..148111 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LDL64_RS29225 (148136) 148136..148336 + 201 WP_072354025.1 hypothetical protein -
LDL64_RS29230 (148356) 148356..149102 + 747 WP_000205770.1 conjugal transfer pilus acetylase TraX -
LDL64_RS29235 (149157) 149157..149717 + 561 WP_000139328.1 fertility inhibition protein FinO -
LDL64_RS29240 (149849) 149849..150049 + 201 WP_015059022.1 hypothetical protein -
LDL64_RS29245 (150435) 150435..151034 + 600 WP_032083981.1 PIN domain-containing protein -
LDL64_RS29250 (151111) 151111..151428 + 318 WP_153449145.1 hypothetical protein -
- (151629) 151629..151688 - 60 NuclAT_1 - Antitoxin
- (151629) 151629..151688 - 60 NuclAT_1 - Antitoxin
- (151629) 151629..151688 - 60 NuclAT_1 - Antitoxin
- (151629) 151629..151688 - 60 NuclAT_1 - Antitoxin
LDL64_RS29255 (151733) 151733..151882 + 150 WP_001312851.1 Hok/Gef family protein Toxin
LDL64_RS29260 (152166) 152166..152414 + 249 WP_000083837.1 replication regulatory protein RepA -
LDL64_RS29265 (152529) 152529..152713 + 185 Protein_207 protein CopA/IncA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 - 1..152725 152725


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T218692 WP_001312851.1 NZ_CP084494:151733-151882 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T218692 NZ_CP116076:41900-42052 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 60 bp

>AT218692 NZ_CP084494:c151688-151629 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C8K5

Antitoxin

Download structure file

References