Detailed information of TA system TA021865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1270831..1271399
• Replicon: chromosome
• Accession: NC_008769
• Organism: Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: BCG_RS06075
• Protein ID: WP_003405865.1
• Coordinates: 1271025..1271399 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: BCG_RS06070
• Protein ID: WP_003405863.1
• Coordinates: 1270831..1271028 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCG_RS06050	1266872..1267510	-	639	WP_011799158.1	lipid droplet-associated protein	-
BCG_RS06055	1267600..1268607	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BCG_RS06060	1268624..1269607	-	984	WP_003898731.1	hypothetical protein	-
BCG_RS06065	1269670..1270743	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
BCG_RS06070	1270831..1271028	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BCG_RS06075	1271025..1271399	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCG_RS06080	1271602..1272300	+	699	WP_010950494.1	hypothetical protein	-
BCG_RS06085	1272418..1272603	+	186	WP_003901093.1	hypothetical protein	-
BCG_RS06090	1272530..1272805	-	276	WP_003405867.1	hypothetical protein	-
BCG_RS06095	1273048..1273371	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
BCG_RS06100	1273386..1274096	-	711	WP_003911399.1	hypothetical protein	-
BCG_RS22495	1274118..1274327	+	210	WP_003911400.1	hypothetical protein	-
BCG_RS06110	1274279..1274986	-	708	Protein_1191	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T21865 WP_003405865.1 NC_008769:1271025-1271399 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T21865 NC_008769:1271025-1271399 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT21865 WP_003405863.1 NC_008769:1270831-1271028 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT21865 NC_008769:1270831-1271028 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07