Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1262075..1262706 Replicon chromosome
Accession NC_008769
Organism Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag BCG_RS06015 Protein ID WP_003405820.1
Coordinates 1262075..1262386 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag BCG_RS06020 Protein ID WP_003405836.1
Coordinates 1262386..1262706 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCG_RS05995 1257557..1258981 - 1425 WP_003405805.1 class II fumarate hydratase -
BCG_RS06000 1259012..1260100 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
BCG_RS06005 1260156..1260800 + 645 WP_174813088.1 DUF4245 domain-containing protein -
BCG_RS06010 1260807..1261963 - 1157 Protein_1172 AI-2E family transporter -
BCG_RS06015 1262075..1262386 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
BCG_RS06020 1262386..1262706 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
BCG_RS20815 1262716..1264241 + 1526 Protein_1175 carboxylesterase/lipase family protein -
BCG_RS06035 1264259..1265371 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
BCG_RS06040 1265381..1265638 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
BCG_RS06045 1265628..1266875 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
BCG_RS06050 1266872..1267510 - 639 WP_011799158.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T21864 WP_003405820.1 NC_008769:c1262386-1262075 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T21864 NC_008769:c1262386-1262075 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT21864 WP_003405836.1 NC_008769:c1262706-1262386 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT21864 NC_008769:c1262706-1262386 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References