Detailed information of TA system TA218057

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 880928..881729
• Replicon: chromosome
• Accession: NZ_CP084096
• Organism: Escherichia coli O78 strain 3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D8EBK0
• Locus tag: LCF29_RS04310
• Protein ID: WP_001094436.1
• Coordinates: 880928..881305 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: LCF29_RS04315
• Protein ID: WP_225343788.1
• Coordinates: 881352..881729 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF29_RS04285 (876081)	876081..877348	-	1268	Protein_843	integrase arm-type DNA-binding domain-containing protein	-
LCF29_RS04290 (877698)	877698..879233	-	1536	WP_000492897.1	EAL domain-containing protein	-
LCF29_RS04295 (879304)	879304..880149	-	846	WP_001280513.1	DUF4942 domain-containing protein	-
LCF29_RS04300 (880234)	880234..880431	-	198	WP_000839254.1	DUF957 domain-containing protein	-
LCF29_RS04305 (880443)	880443..880931	-	489	WP_000761714.1	DUF5983 family protein	-
LCF29_RS04310 (880928)	880928..881305	-	378	WP_001094436.1	TA system toxin CbtA family protein	Toxin
LCF29_RS04315 (881352)	881352..881729	-	378	WP_225343788.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
LCF29_RS04320 (881808)	881808..882029	-	222	WP_000692312.1	DUF987 domain-containing protein	-
LCF29_RS04325 (882098)	882098..882574	-	477	WP_001186756.1	RadC family protein	-
LCF29_RS04330 (882589)	882589..883074	-	486	WP_000860054.1	antirestriction protein	-
LCF29_RS04335 (883165)	883165..883983	-	819	WP_001175142.1	DUF932 domain-containing protein	-
LCF29_RS04340 (884073)	884073..884306	-	234	WP_001278287.1	DUF905 family protein	-
LCF29_RS04345 (884312)	884312..884989	-	678	WP_001097312.1	hypothetical protein	-
LCF29_RS04350 (885137)	885137..885817	-	681	WP_001282927.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13958.84 Da
• Isoelectric Point: 6.8603

>T218057 WP_001094436.1 NZ_CP084096:c881305-880928 [Escherichia coli O78]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T218057 NZ_CP115447:c2645960-2645565 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13796.62 Da
• Isoelectric Point: 6.2021

>AT218057 WP_225343788.1 NZ_CP084096:c881729-881352 [Escherichia coli O78]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQESNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT218057 NZ_CP115447:c2646202-2645957 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• AlphaFold DB: A0A0E2L1N0