Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 64513..65114 Replicon plasmid pX14-3-tetA
Accession NZ_CP084056
Organism Escherichia coli strain X14-3

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag LCR13_RS22600 Protein ID WP_001216034.1
Coordinates 64513..64893 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag LCR13_RS22605 Protein ID WP_001190712.1
Coordinates 64893..65114 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCR13_RS22575 (LCR13_22585) 59954..61396 - 1443 WP_223656860.1 terminase -
LCR13_RS22580 (LCR13_22590) 61438..62631 - 1194 WP_032214401.1 hypothetical protein -
LCR13_RS22585 (LCR13_22595) 62717..63169 - 453 WP_001326849.1 late promoter-activating protein -
LCR13_RS22590 (LCR13_22600) 63258..64301 - 1044 WP_094318254.1 DUF968 domain-containing protein -
LCR13_RS22595 (LCR13_22605) 64329..64508 - 180 WP_000113018.1 hypothetical protein -
LCR13_RS22600 (LCR13_22610) 64513..64893 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LCR13_RS22605 (LCR13_22615) 64893..65114 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LCR13_RS22610 (LCR13_22620) 65187..65576 - 390 WP_000506724.1 S24 family peptidase -
LCR13_RS22615 (LCR13_22625) 65928..66335 + 408 WP_223656859.1 hypothetical protein -
LCR13_RS22620 (LCR13_22630) 66336..66692 + 357 WP_001062545.1 hypothetical protein -
LCR13_RS22625 (LCR13_22635) 67768..67998 - 231 WP_223656858.1 hypothetical protein -
LCR13_RS22630 (LCR13_22640) 68126..69304 - 1179 WP_094318253.1 hypothetical protein -
LCR13_RS24390 69314..69448 - 135 WP_272955174.1 hypothetical protein -
LCR13_RS22635 (LCR13_22645) 69499..70005 - 507 WP_000021754.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid floR / tet(A) / qnrS1 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id - 1..108559 108559


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T218051 WP_001216034.1 NZ_CP084056:c64893-64513 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T218051 NZ_CP115447:c2142156-2141722 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCGCAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT218051 WP_001190712.1 NZ_CP084056:c65114-64893 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT218051 NZ_CP115447:c2142417-2142163 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References