Detailed information of TA system TA217958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 976061..977498
• Replicon: chromosome
• Accession: NZ_CP084026
• Organism: Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: LC440_RS04785
• Protein ID: WP_000155570.1
• Coordinates: 976425..977498 (-)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: LC440_RS04780
• Protein ID: WP_000461704.1
• Coordinates: 976061..976432 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC440_RS04745 (971262)	971262..971414	+	153	Protein_923	arsenate reductase	-
LC440_RS04750 (971801)	971801..972268	-	468	WP_000702495.1	protein YfjT	-
LC440_RS04755 (972292)	972292..972735	-	444	WP_000824222.1	lipoprotein YfjS	-
LC440_RS04760 (972735)	972735..972971	-	237	WP_001144029.1	protein YpjK	-
LC440_RS04765 (973012)	973012..973713	-	702	WP_000189409.1	WYL domain-containing protein	-
LC440_RS04770 (973930)	973930..974751	-	822	WP_000197391.1	DUF932 domain-containing protein	-
LC440_RS04775 (974843)	974843..975706	-	864	WP_000787597.1	GTPase family protein	-
LC440_RS04780 (976061)	976061..976432	-	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
LC440_RS04785 (976425)	976425..977498	-	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
LC440_RS04790 (977640)	977640..977903	+	264	WP_000502050.1	DUF1524 domain-containing protein	-
LC440_RS04795 (978263)	978263..979879	+	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
LC440_RS04800 (979876)	979876..982065	+	2190	WP_000136402.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	luxS	911300..987257	75957

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T217958 WP_000155570.1 NZ_CP084026:c977498-976425 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T217958 NZ_CP114896:965796-965972 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT217958 WP_000461704.1 NZ_CP084026:c976432-976061 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT217958 NZ_CP114896:966018-966434 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• AlphaFold DB: A0A373F4G4